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Staining - PowerPoint PPT Presentation

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Staining
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  1. Staining • Examination of malarial parasite. • The blood is stained with Wright's stain. • An ordinary blood smear, if parasite are present ,they may be easily recognised . Sometimes. • Thick blood smear-> present of org. • Thin blood smear -> spp . identification

  2. Plasmodium falciparum

  3. Tertian malaria • Plasmodium vivax • Small ring body , soon become pigmented. • Enlarged (3 to 4 times) • Thin ring of protoplasm showing around the body of the parasite. • Granules distributed all through it. • Red blood cells swell to the large size.

  4. Quartan malaria • Plasmodium malaria • Small ring ,soon become larger and pigmented granules , arranged around the periphery of org. • Red blood cell do not swell to the large size. • Even shrinking a little.

  5. Estivo Autumnal malaria • Plasmodium falciparum • Small ring, it is thicker in one side than the other .so called “signet ring’

  6. Red blood cells • Red blood cells are the principle sites of infection in malaria. • The growing parasite consumes and degrades the intracellular protein , mainly hemoglobin . • The transport properties of the red cells membrane are altered , cryptic surface antigen are exposed and new parasite derived protein are inserted . • In P . infection ,membrane portuerance appear on the red cell surface in the second 24 hour of the asexual cycle . • histidine – rich parasite protein are found under these “knobs ” . • These knobs extrude a strain specific , adhesive variant protein of high molecular weight that mediates red cell attachment to receptors on venular and capillary endothelial , causing cytoadherence . P . falciparum infected red cells also adhere to uninfected red cells to form rosettes . • Cytoadherence and rosetting are central to the pathogenesis of P . falciparum malaria , resulting in the formation of red cell aggregates and intra vascular sequestration of red cells in the vital organs like the brain and heart .

  7. Malaria (Plasmodium falciparum)

  8. Malarial parasite(singnet ring)

  9. Life Cycle of the Malaria Parasite A female Anopheles mosquito carrying malaria-causing parasites feeds on a human and injects the parasites in the form of sporozoites into the bloodstream. The sporozoites travel to the liver and invade liver cells. Over 5-16 days, the sporozoites grow, divide, and produce tens of thousands of haploid forms, called merozoites, per liver cell. Some malaria parasite species remain dormant for extended periods in the liver, causing relapses weeks or months later. The merozoites exit the liver cells and re-enter the bloodstream, beginning a cycle of invasion of red blood cells, asexual replication, and release of newly formed merozoites from the red blood cells repeatedly over 1-3 days. This multiplication can result in thousands of parasite-infected cells in the host bloodstream, leading to illness and complications of malaria that can last for months if not treated. Some of the merozoite-infected blood cells leave the cycle of asexual multiplication. Instead of replicating, the merozoites in these cells develop into sexual forms of the parasite, called male and female gametocytes, that circulate in the bloodstream.  When a mosquito bites an infected human, it ingests the gametocytes. In the mosquito gut, the infected human blood cells burst, releasing the gametocytes, which develop further into mature sex cells called gametes. Male and female gametes fuse to form diploid zygotes, which develop into actively moving ookinetes that burrow into the mosquito midgut wall and form oocysts.  Growth and division of each oocyst produces thousands of active haploid forms called sporozoites. After 8-15 days , the oocyst bursts, releasing sporozoites into the body cavity of the mosquito, from which they travel to and invade the mosquito salivary glands. The cycle of human infection re-starts when the mosquito takes a blood meal, injecting the sporozoites from its salivary glands into the human bloodstream