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STAINING

STAINING. The process of adding a dye to a bacterial culture. Dyes. Basic dye—possess a positive charge Acidic dye—possess a negative charge Remember, bacteria posses a slight negative charge on their surface. SIMPLE STAINING. Use only one dye

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STAINING

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  1. STAINING The process of adding a dye to a bacterial culture

  2. Dyes • Basic dye—possess a positive charge • Acidic dye—possess a negative charge Remember, bacteria posses a slight negative charge on their surface

  3. SIMPLE STAINING • Use only one dye • For the purpose of viewing bacterial shapes and arrangements • Simple Stain the following: 1) E. coli 2) S. aureus 3) A colony from the “Microbes in the Environment” plate

  4. 3 Types of Staining Procedures • Simple Staining (shapes and arrangements) • Differential Staining (Gram reactions) • Special Staining (Capsule, flagella, spores)

  5. PROCEDURE: • Prepare smear of bacterium • Air dry • Heat fix the bacteria to the slide (release of “sticky” proteins from the cell surface of the bacteria adheres the bacterial cell to the slide) • Apply crystal violet to the smear; let stand 45 seconds

  6. Procedure Cont. • Rinse with distilled water or tap water • Blot dry with bibulous paper • View using microscope

  7. Page 49

  8. Page 54

  9. Methylene blue Crystal Violet Fuchsin Simple stains allow visualization of Shapes Arrangements Proteus vulgaris Staphyloccocus aureus Bacillus cereus Page 55

  10. Fracisella tularensis Causitive agent of Rabbit fever Methylene blue stain Sacharromyces cerevisiae (Brewer’s yeast) Methylene blue stain

  11. Bacillus anthracis (anthrax) Gram positive Crystal violet stain Campylobacter jejuni (traveler’s diarrhea) Gram negative Fuchsin stain

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