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Root Phenotyping for Water Response in Clonal Forestry: Study of Elite Families

Explore the importance of root growth in seedling establishment under water deficits. Molecular markers and WUE traits are key for selection. Follow the FBRC.CCLONES experiment, tracking 60 clones per elite family in 15 families across different field locations. Phenotyping includes root biomass, architecture, shoot height, survival, and disease incidence measurements. Additional physiological measurements focus on specific leaf area, 12C:13C ratios, water potential, and RWC during water deficit periods.

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Root Phenotyping for Water Response in Clonal Forestry: Study of Elite Families

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  1. Phenotyping: Response to Water Deficits • Motivation • Importance of root growth to seedling establishment • Water is widespread limitation to productivity • Water limitations common in seedling stage • Little gene discovery work with roots • Molecular markers for root traits have great potential for application • WUE identified as selection trait for other species • Growing importance of clonal forestry for industry

  2. FBRC CCLONES Experiment • 60 clones from each of 15 elite, full-sib families • 8 ramets/clone planted at each of six field locations by FBRC cooperators in December 2002 • Two cultural treatments: "optimal" and low-intensity

  3. Phenotyping Measurements - FBRC CCLONES population • Destructive root biomass and architecture measurements on 4 ramets/clone prior to planting (Dec 2002) • Measurements 1 year post-planting, all sites (21,600 plants) • shoot height • survival • disease incidence

  4. Phenotyping Measurements - FBRC CCLONES population • Additional physiological measurements 1 year post-planting (2003) • Intensive blocks, two sites with contrasting soils, subset of 600 clones (4800 plants) • specific leaf area • late-summer foliar 12C:13C • pre-dawn water potential and RWC during water deficit periods in May and October

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