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January 25, 2012. Introductions Chapter 8 Concepts Chapter 8 Terms Intro to plant cloning Assignment: Terms. Define terms on page 171 Due tomorrow! (Thursday) Reading: Ch 8. Intros!. Mrs. Haddad (Mrs. H) UW-Platteville Agricultural Education Biotechnology I like to:
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January 25, 2012 • Introductions • Chapter 8 Concepts • Chapter 8 Terms • Intro to plant cloning • Assignment: Terms. Define terms on page 171 • Due tomorrow! (Thursday) • Reading: Ch 8
Intros! • Mrs. Haddad (Mrs. H) • UW-Platteville • Agricultural Education • Biotechnology • I like to: • Travel, play sports, scrapbook, and show cows • mrshonlineag.wordpress.com
But First… http://www.youtube.com/watch?v=jvnKybI7fzU
Key Concepts Plants naturally propagate by cloning Advantages of cloning Identify plant “anatomy” involved Describe various methods Role of plant hormones Genetic alteration
Key Terms • Asexual Reproduction • Type of cloning that ensures a desirable species would be produced • Vegetative Propagation • Increase in the number of plants by planting seeds, using cuttings, division, grafting or layering • Plastids • Plant cell bodies containing photosynthetic pigments
Key Terms • Separation • Pulling apart plants where they naturally separate for propagations • Division • Plant cuttings into sections and growing new from each section • Stolons • Runners; specialized stems that branch out horizontally above ground
Key Terms • Rhizomes • Elongated underground stems; often tuber shaped • Tuber • Thickened or swollen underground branch or stolon with numerous buds • Bulbs • Subterranean buds with overlapping membrane-like leaf bases
Key Terms • Tunicate • Bulb with dry outer layers and grows via small “bublets” • Nontunicate • Bulb with layers of outer scales that can be separated and propagated • Corms • Enlarged fleshy base of a stem in which food accumulates
Key Terms • Auxin • Plant hormones • Layering • Propagations via covering a portion of the plant with soil to encourage rooting off a stem of the parent plant • Grafting • Plant material from two separate plants joined into one
Key Terms • Scion • In grafting, the upper part of the plant • Cambium • Actively growing cells between bark and wood • Callus Cells • Undifferentiated tissue cells
Key Terms • Xylem • Plant “plumbing;” channel for water and dissolved minerals • Phloem • Inner bark; channel for food throughout the plant • Tissue Culture • Small amount of tissue used to grow a new plant
Key Terms • Meristem • Plant tissue with undifferentiated cells • Explant • Plant tissue containing meristem cells; taken from very end of stem or root
Intro to Plant Cloning • History: • One of the oldest forms of cloning • Natural • Man made • Asexual Reproduction • Vegetative Propagation • STRAWBERRIES!
January 26, 2012 Term Review FREE WRITE Discussion Advantages of Plant Cloning Assignment: Lab Write Up TOMORROW: Lab!
Advantages of Plant Cloning • FREE WRITE! • Take three minutes to list all of the advantages of plant cloning you can think of.
Advantages of Cloning • Video • http://www.youtube.com/watch?v=bWOAPYcMPHw
Advantages of Cloning Genetically identical More efficient Seedless Uniformity Resistance Yield Fertility Increased possibilities Nutritional Benefits Dependability
Disadvantages Genetically identical Diversity Disease Spread Disease Susceptibility Cost Time Reliance on humans Ethics
According to the USDA… • Yield • A cloned plant can yield a thousand new plants from one parent plant. This means that farmers can produce more crops without a lot of seeds. • Strength • Cloning a plant means you can choose the best plants to clone. An entire crop of healthy, prosperous plants can be cloned from one strong parent plant. • Timing • Cloned plants grow at the same rate, so harvesting can become streamlined. • Food Options • So far, the FDA has stated that cloned food would be perfectly safe for humans to eat. They have not, however, finalized a ruling to allow cloned food on the market. Read more: What Are the Advantages of Cloning in Agriculture? | eHow.comhttp://www.ehow.com/facts_5750461_advantages-cloning-agriculture_.html#ixzz1kU9V5qZV
In Preparation for Lab • Fingerprinting & DNA Extraction • DNA=code of life • It’s how we know that there are over one million differences between you and me. (That’s 0.1% of our DNA!!!) • Read Introduction
January 27, 2012 • LAB! • DNA Extraction and Fingerprinting • Monday: • Video: The Future of Food • Prepare for critical thinking!!
February 1, 2012 • Types of cloning • Tomorrow: • Packet Due! • Current Events work day • Meet here!
Types of Cloning Separation & Division Tuber & Bulbs Cuttings Layering Grafting Tissue Culture
But First… • A few differences between animal & plant cells • Plants: Central Vacuole, Cell Wall, Plastids • EASIER TO CLONE!! • Why? • Greater natural frequency • Mitosis vs Meiosis
Separation and Division • Separation • Genetic consistency • Faster growth • Hybrid options • Division • Plant rejuvenation • Early spring
Tubers and Bulbs • Tubers • Uniform crop vs True Seed • New vs Old • Emergence • Stems • Tuber initiation • Maturity • Vigor • Yield • Bulbs • Underground • Divide into two
Cuttings • Cut depends on plant species • Ex: Potato • http://www.ehow.com/video_7618017_plant-rose-bush-cuttings.html • He’ll explain it better than I will…
Layering • Three Types: • Air (Ex: Rubber Plant) • Mound (Ex: Shrubs) • Tip (Ex: Black Raspberries) • Bury a branch in the soil • Eventually form roots • Cut from original • Transplant
Grafting • A scion is removed from one plant and fused onto another plant • Cleft • The stock is cut in half, perpendicular to the ground. The top is split, the scion inserted, and the graft sealed with special wax. • Bark • The end of the scion is cut into a thin wedge, and the scion is stored in a refrigerator. The scion is inserted under the bark, secured with wire staples and sealed with wax. • Whip/Tongue • Scion and stock are of the same diameter. A slender V cut is made at the end of the scion and a matching V is cut into the stock. The point of the scion is placed into the receiving V and the graft is bound and sealed with wax. • Best time for a woody cut is during dormancy!!
Tissue Culture Originated in France (mid ‘60s) Micro propagation (In-Vitro) Screen cells rather than plants Produce valuable compounds Cross distant species Rapid breeding Tissue for transformation Obtain “clean stock” from meristem Large numbers of identical individuals
Fun Facts • Some plants grown only for the male • Ex: Asparagus • Some are propagated by their rhizomes & corms • Bermuda Grass • Gladiolus • High relative humidity helps growth after propagation
February 2, 2012 Packet Due TODAY! Cloning GMOs Packet Due Today! Friday: Terms Quiz & Current Events
Article Read the article Answer the questions Highlight words or phrases you don’t understand
February 3, 2012 • Terms Quiz • Current Events • Think of questions • Agronomy speaker Monday!!
February 6, 2012 • Today: Agronomy Speaker • Tomorrow: LAB • Tonight: Be ready for lab quiz before lab tomorrow!
February 7, 2012 LAB! Tomorrow: Current Events presentations
February 8, 2012 Test Review TEST TOMORROW!!