Domains 1 and 2 (D1/D2) of LSU rDNA. Easy to PCR (universal primers), sequence, align… Short (400-650 bp) but variable enough to distinguish most of yeast species Universally available database for all known yeast species Kurtzman and Robnett (1998) - ascomycete yeasts
Rapid ID of yeasts by gene sequences
divergence among ascomycetous yeasts
Strain pairs % nDNA D1/D2 nucleotide
Conspecific >70% 0-3
Unrelated <20% 6-250
Kurtzman 2006, Mycoscience 47: 65-71
686 strains - Known yeasts
81 ascomycetous spp.
39 basidiomycetous spp.
1241 strains - New yeasts
214 ascomycete spp.
31 basidiomycete spp.
618 strains - Described
as 66 novel ascomycete spp.
1927 yeast strains from insect guts
Is single gene enough for ID?