Domains 1 and 2 (D1/D2) of LSU rDNA
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Domains 1 and 2 (D1/D2) of LSU rDNA. Easy to PCR (universal primers), sequence, align… Short (400-650 bp) but variable enough to distinguish most of yeast species Universally available database for all known yeast species Kurtzman and Robnett (1998) - ascomycete yeasts

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Rapid id of yeasts by gene sequences

  • Easy to PCR (universal primers), sequence, align…

  • Short (400-650 bp) but variable enough to

  • distinguish most of yeast species

  • Universally available database for all known yeast species

  • Kurtzman and Robnett (1998) - ascomycete yeasts

  • Fell et al. (2000) - basidiomycete yeasts

Rapid ID of yeasts by gene sequences


Rapid id of yeasts by gene sequences

Correlation of nDNA relatedness and D1/D2 sequence

divergence among ascomycetous yeasts

Strain pairs % nDNA D1/D2 nucleotide

relatedness differences

Conspecific >70% 0-3

Unrelated <20% 6-250

Kurtzman 2006, Mycoscience 47: 65-71


Rapid id of yeasts by gene sequences

D1/D2 sequencing

Database

686 strains - Known yeasts

81 ascomycetous spp.

39 basidiomycetous spp.

1241 strains - New yeasts

214 ascomycete spp.

31 basidiomycete spp.

18S, ITS,

phenotypic characters

618 strains - Described

as 66 novel ascomycete spp.

1927 yeast strains from insect guts


Rapid id of yeasts by gene sequences

C

Is single gene enough for ID?

B

A


Rapid id of yeasts by gene sequences

  • Other genes

  • ITS - widely, useful for closely related taxa

  • cytochrome oxidase II - Belloch et al. 2000

  • actin-1 - Daniel et al. 2001

  • EF1-a, RPB II, mSSU - Kurtzman & Robnett 2003, 2007