Rapid Identification of Yeasts - PowerPoint PPT Presentation

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Rapid Identification of Yeasts

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  1. Rapid Identification of Yeasts

  2. Rapid Identification

  3. Two Alternative Approaches

  4. Aim and Methodology

  5. Topics to be covered


  6. Saccharomyces species-specific

  7. 2011

  8. Discovery of S. eubayanus*

  9. S.eubayanus species-specific primers have been developed (228bp)

  10. S. eubayanus with hybrid strains

  11. Multiplex False Positives

  12. Summary

  13. Genus-specific primers

  14. A. Use alignments (as for species-specific)

  15. B. Look for regions within Saccharomyces ORFS that are missing in outgroups

  16. Sec24p structure

  17. C. Use genes absent in non-Saccharomyces speciesYeast Gene Order Browser (YGOB)

  18. Results

  19. Conclusions

  20. Zygosaccharomyces species

  21. Species-specific Colony PCR

  22. Z. siamensis

  23. Zygosaccharomyces Genus-specific Primers

  24. Zygosaccharomyces Genus-specific Primers

  25. Conclusions for Zygosaccharomyces

  26. Level of detection in liquid cultures

  27. Limitations of the Method

  28. Overall Advantages

  29. Conclusions

  30. Acknowledgements

  31. Identification by Molecular Methods

  32. Hybrid detection in S. pastorianus

  33. Hybrid detection in S. pastorianus

  34. Hybrid detection in S. pastorianus

  35. Hybrid detection in S. pastorianus

  36. Current Saccharomyces species

  37. Tests against a diverse global collectionS. paradoxus specific primers