1 / 27

NMR Microscopy

NMR Microscopy. Stef VanGorden Joseph Hornak Rochester Institute of Technology. Objective. The main goal of this research was to create a NMR microscope capable of producing a 1 mm thick, approximately 112 micron in-plane resolution tomographic images. Bruker 300 DRX Spectrometer.

melosa
Download Presentation

NMR Microscopy

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. NMR Microscopy Stef VanGorden Joseph Hornak Rochester Institute of Technology

  2. Objective • The main goal of this research was to create a NMR microscope capable of producing a 1 mm thick, approximately 112 micron in-plane resolution tomographic images. • Bruker 300 DRX Spectrometer

  3. Overview of my Presentation • Background • Experimental • Applications • Conclusions

  4. BACKGROUND

  5. n = g B g = 42.58 MHz/T E = hn = hgB Energy Level Diagram (Hornak 1997)

  6. Gy Sample with a Uniform Field N HydrogenMolecules S (Hornak 1997)

  7. Gy Sample with Increasing Gradient HydrogenMolecules n = g B = g x Gx (Hornak 1997)

  8. EXPERIMENTAL

  9. Starting Point • Reproduction of previous 1-D and 2-D imaging results. • Slice Selective Sequence Evaluation

  10. Slice Selective Sequence Gz Gy Gx Slice Selective Case (tomographic slice)

  11. Timing Diagram for the Slice Selective NMR Microscope

  12. Variables THK = 2/(gGztw) Thick Slice (From ~1mm to ~10 mm) Width of 90° pulse = 80 µs tw (180°) =160 µs Amplitude attenuation = 20 dB Thin Slice (From ~.5 mm to ~1 mm) Width of 90° pulse = 157 µs tw (180°) = 314 µs Amplitude attenuation = 26 dB

  13. Applying Gradients THK = 2/(gGztw) 100% =50 G/cm Maximum 10% = 5 G/cm => 6 mm 60% = 30 G/cm => 1 mm

  14. Feature Documentation In-plane Resolution Phantoms Slice Thickness Phantoms

  15. In-plane Resolution

  16. In-plane Resolution 284 mm 112 mm Fishing line in capillarytubes Glass rods

  17. In-plane Resolution 45 mm -- 112 mm object --- image 1 pixel = 23.7 mm

  18. Slice Thickness Phantom Screw phantom in sample tube Signal from the screw phantom. THK = (f/360) (1/32 in/threads)(25.4 mm/in) THK = .8 mm for an angle of 360°

  19. Nylon Screw Phantom Images x x y y z y One or more rotations x x y y Less than one rotation

  20. Cone Phantom Design Illustration of Cone Phantom

  21. Cone Phantom Images Images with slice thickness of 1mm and .5 mm respectively. .711 mm

  22. Analyzing Wedge Images .55mm 4° 4.25mm Portion of Sample Tube (profile of middle)

  23. APPLICATIONS

  24. Celery Images Thick slice Thinner slice Thinnest slice Day 1: Images Two Weeks Later

  25. House Fly Image

  26. Conclusion • Moved practical NMR microscopy at RIT closer to reality. • Current Capabilities slice thickness ~.5 mm in-plane resolution ~ 100 microns

  27. y x The Next Step ... • Slice Selection Locator • SNR improvements • Further In-plane Resolution Evaluation Slice Selection Locator

More Related