Voltage clamp
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Voltage Clamp. Patch Clamp Applications. Evidence that potassium is primarily responsible for the RMP. Neuron membrane is at rest. Neuron membrane is at rest. Now let’s look at the responses of a neuron to various stimuli.

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Slide11 l.jpg


Normal membrane current during the action potential l.jpg
Normal membrane current during the action potential. during depolarization phase of the action potential. Use of the voltage clamp to measure current.


Membrane current when there is no na contribution l.jpg
Membrane current when there is no Na contribution. during depolarization phase of the action potential. Use of the voltage clamp to measure current.


Combine these two curves l.jpg
Combine these two curves during depolarization phase of the action potential. Use of the voltage clamp to measure current.




Slide17 l.jpg
These curves are all “normal” curves during action potential, with the membrane held at various voltages by the voltage clamp.


E na 50 mv in this experiment l.jpg
E potential, with the membrane held at various voltages by the voltage clamp.Na+ ~ 50 mV in this experiment


This diagram is similar to fig 5 21 l.jpg
This diagram is similar to Fig. 5.21 potential, with the membrane held at various voltages by the voltage clamp.



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