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DNA Fingerprinting Gel Staining and Analysis Student Instructions

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DNA Fingerprinting Gel Staining and Analysis Student Instructions

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    1. DNA Fingerprinting Gel Staining and Analysis Student Instructions

    2. Gel Staining and Analysis Step 1 When electrophoresis is completed, turn off the power supply. Remove the lid from the box. Lift the lid to remove it from the box.

    3. Gel Staining and Analysis Step 2 To stain the gel to make the DNA band visible, remove the gel tray from the electrophoresis unit and place the tray into a plastic container. Slide the gel off the tray by pushing on one end of it.

    4. Gel Staining and Analysis Step 3 Add the Carolina Blu DNA Stain, making sure the gel is completely immersed. Do not pour the stain directly on the gel. Stain the gel for 15 minutes. Agitate gently, if possible.

    5. Gel Staining and Analysis Step 4 Pour the stain back into the bottle. Cover the gel with distilled water to destain. Agitate gently, if possible, for 30 to 40 minutes, changing the water every 10 minutes. (Tap water contains chloride ions that can partially remove the stain from the DNA bands and give inferior results.) Agitate gently, if possible. During the 30 to 40 minutes of destaining, change the water every 10 minutes, if possible. During the destaining process, the bands of DNA will become more clear as the stain is removed from the remainder of the gel. It is possible to destain the gel for up to 24 hours. If the DNA bands become too light, the gel can be stained and destained again(Tap water contains chloride ions that can partially remove the stain from the DNA bands and give inferior results.) Agitate gently, if possible. During the 30 to 40 minutes of destaining, change the water every 10 minutes, if possible. During the destaining process, the bands of DNA will become more clear as the stain is removed from the remainder of the gel. It is possible to destain the gel for up to 24 hours. If the DNA bands become too light, the gel can be stained and destained again

    6. Gel Staining and Analysis Step 5 The gel can be displayed to the class by sliding it onto a piece of plexiglass and placing it on a white sheet of paper or on a white-light viewing box. The gel can be saved for at least a month in a refrigerator by sliding it into a clear plastic bag and sealing the bag. The gel can be viewed on a white sheet of paper or a white-light viewing box without removing it from the bag. None of the chemicals used in the experiment are toxic. Solutions can be poured down a conventional drain. The gel can be disposed of with other trash.The gel can be saved for at least a month in a refrigerator by sliding it into a clear plastic bag and sealing the bag. The gel can be viewed on a white sheet of paper or a white-light viewing box without removing it from the bag. None of the chemicals used in the experiment are toxic. Solutions can be poured down a conventional drain. The gel can be disposed of with other trash.

    7. Gel Staining and Analysis Step 6 Ideal gel Stained with ethidium bromide and viewed under UV light source

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