enterotoxigenic bacteroides fragilis etbf l.
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新衍生的食品中毒細菌 ENTEROTOXIGENIC BACTEROIDES FRAGILIS (ETBF) PowerPoint Presentation
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新衍生的食品中毒細菌 ENTEROTOXIGENIC BACTEROIDES FRAGILIS (ETBF). 特性. 格蘭式陰性短桿菌 不產孢子 厭氧 毒素是 Bacteroides fragilis toxin(BFT) ( 一種 extracellular heat-labile protein toxin) 作用在細胞骨架,使腸道表皮細胞變形. Bacteroides fragilis toxin(BFT). 1983 年在羊群腹瀉的糞便中發現 , 刺激羊的結腸的分泌反應 1987 年在急性腹瀉成人和小孩的糞便中發現 依據能否分泌 BFT 來將菌種分類為

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slide2
特性
  • 格蘭式陰性短桿菌
  • 不產孢子
  • 厭氧
  • 毒素是Bacteroides fragilis toxin(BFT)

(一種extracellular heat-labile protein toxin)

  • 作用在細胞骨架,使腸道表皮細胞變形
bacteroides fragilis toxin bft
Bacteroides fragilis toxin(BFT)
  • 1983年在羊群腹瀉的糞便中發現,刺激羊的結腸的分泌反應
  • 1987年在急性腹瀉成人和小孩的糞便中發現
  • 依據能否分泌BFT來將菌種分類為

enterotoxigenic Bacteroides fragilis(ETBF)

nonenterotoxigenic Bacteroides fragilis(NTBF)

  • 有3種BFT異構體,即BFT-1 ,BFT-2 ,BFT-3
slide4
致病性
  • 除了家畜外,人類也會感染
  • 成人大多感染的是bft-1
  • 兒童大多感染的是bft-2(其中1-5歲幼兒腹瀉感染關係較為密切)
  • 也可能致癌
  • 致病機制:改變纖維肌動蛋白的結構,裂解黏著小蛋白(E-鈣黏素),導致腸上皮細胞的緊密聯繫被破壞增加了通透性
slide5
鑑定&耐受性
  • 用 primers 404/407 和PCR 去鑑定B. fragilis enterotoxin (fragilysin) 基因
  • 菌株對抗生素的耐受性由藥物敏感性測試(Etest)決定

(對amoxicillin/clavulanic acid, imipenem and metronidazole是 susceptible )

  • 在體外(in vivo)實驗中, 和 beta-lactamase inhibitors, carbapenems and metronidazole結合的beta-lactams 最有效用
slide7

Tumors in the colons of multiple intestinal neoplasia (Min) mice colonized with enterotoxigenic Bacteroides fragilis (ETBF) for 3 months

compared to Min mice not colonized with ETBF. The colons are stained with methylene blue.

references
References:
  • CAT.INIST Cat.inist or about 15 million bibliographic records (back to 1973) of documents held in the INIST/CNRS collections and covering all fields of worldwide research in Science, Technology, Medicine, Humanities and Social Sciences.
  • http://cat.inist.fr/?aModele=afficheN&cpsidt=3582450
  • Myers LL, Shoop DS, Stackhouse LL, Newman FS, Flaherty RJ, Letson GW, Sack RB. Isolation of enterotoxigenic Bacteroides fragilis from humans with diarrhea.J Clin Microbiol. 1987 Dec;25(12):2330-3.
  • Vu Nguyen T, Le Van P, Le Huy C, Weintraub A.Diarrhea caused by enterotoxigenic Bacteroides fragilis in children less than 5 years of age in Hanoi, Vietnam.Anaerobe. 2005 Feb-Apr;11(1-2):109-14. Epub 2004 Dec 9.
  • http://www.blackwellpublishing.com/eccmid14/abstract.asp?id=14528
  • http://www.cqvip.com/onlineread/onlineread.asp?ID=15949818&SUID=EGBNBFDHDNCBCOPIBOLGEMCBCDOIPNFP#