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New Wine in Old Bottles A New Approach of SNP Function Research. Soochow University. Yifeng Zhou Ph.D. zhouyifeng@suda.edu.cn. Why We Design This Study?. An Unfinished Story: SNP in TIMP2 Promoters and Risk of Breast Cancer. 1. What Did We Publish Before ?. 2.

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Presentation Transcript
yifeng zhou ph d

New Wine in Old BottlesA New Approach of SNP Function Research

Soochow University

Yifeng Zhou Ph.D

zhouyifeng@suda.edu.cn

why we design this study
Why We Design This Study?

An Unfinished Story: SNP in TIMP2 Promoters and Risk of Breast Cancer

1

What Did We Publish Before?

2

What Did We not Publish Before?

What Did We Perform Now?

3

What Did We Publish Now?

4

my first paper
My First Paper

Zhou et al.Carcinogenesis. 2004 25:399-404.

backgroud
Backgroud

TIMP-2: An Endogenous Inhibitor

of MMP-2

TIMP-2 binds with Pro-MMP-2

result i
Result I

Zhou et al.Carcinogenesis. 2004 25:399-404.

result ii
Result II

Zhou et al.Carcinogenesis. 2004 25:399-404.

function assays of snps in promoter

SNPs in

Promoter

Real-Time

PCR…

EMSA

Report

Gene

ChIP

Function Assays of SNPs in Promoter
slide8
EMSA

Data Unpublished

mechanism
Mechanism

Aishan HAN et al. ANALYTICAL SCIENCES 2006

mechanism1
Mechanism

Huan Chen et al. Biosensors and Bioelectronics 24 2009 1955–1961

material
Material

Graphite

Glassy Carbon

results
Results
  • Cyclic voltammograms (scan rate:100mVs-1)
  • (b) Electrochemical impedance spectroscopy (Nyquist plots) response at different modified process in the electrode
results2
Results

Differential pulse voltammograms of [Fe(CN)6]3-/4-accumulated on the

probe after its interaction with different concentration of the wtDNA

results4
Results

The washing ratio responses as a function of NaCl concentrations in washing buffer

results6
Results

EMSA

Report Gene

G Allele significant higher than C Allele

No Difference Between two Allele

TIMP-2 -418G/C

Breast Cancer

Electrochemistry

Case-Control

Affinity of G is significant higher than C

P = 0.060

conclusion
Conclusion
  • Present study provides further evidence that the functional SNP in TIMP-2 promoter is genetic susceptibility factors for breast cancer.
publications
Publications

Gao Y et al. Carcinogenesis. 2009 30:2064-9.

acknowledgment
Yu Xiao

Jiang Lan

Zheng Jian

Kang Shenhui

Peng Rui

Xu Jinhai

Acknowledgment
thank you

Thank You!

zhouyifeng@suda.edu.cn