Supplementary Figure 1. AMD/LPS-interaction caused hepatotoxicity in mice

1. Tumor necrosis factor-alpha potentiates the cytotoxicity of amiodarone in Hepa1c1c7 cells: roles of caspase activation and oxidative stress Supplementary Figures Jingtao Lu*†, Kazuhisa Miyakawa‡†, Robert A. Roth§† and Patricia E. Ganey§†,1. *Department of Biochemistry and Molecular Biology, †Center for Integrative Toxicology, ‡Department of Pathobiology and Diagnostic Investigation, §Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA 1 To whom correspondence should be addressed at Department of Pharmacology and Toxicology, Michigan State University, 214 Food Safety and Toxicology Building, East Lansing, MI 48824. Fax: (517) 432-2310. E-mail ganey@msu.edu.

2. * # Supplementary Figure 1. AMD/LPS-interaction caused hepatotoxicity in mice Mice were treated with LPS (2×106 EU/kg, ip) or saline and 2h later with AMD (300 mg/kg, ip) or veh. Plasma ALT activity was measured at 24h after LPS. Two-way ANOVA was applied. #, significantly different from respective groups not given LPS; * significantly different from respective group not given AMD. Significant interaction were observed between AMD and LPS p< 0.05, n=3-6.

3. Supplementary Figure 2. TNF potentiation of AMD cytotoxicity in HepG2 cells HepG2 cells were treated with AMD (40uM to 125uM) or saline and TNF (1 ug/ml) or saline. After 24h incubation, the release of LDH was determined as described in Materials and Methods. n=2.

4. Supplementary Figure 3. Activity of caspase 8 after AMD and/or TNF treatment Hepa1c1c7 cells were treated with 35μM AMD and/or 3ng/ml TNF for 1h, 12h, 24h or 48h, and activity of caspase 8 was measured as described in Materials and Methods. Results were presented as fold changes from Sal/Sal group. n=3-5. No significant differences were observed.