entamoeba histolytica n.
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Entamoeba histolytica. Differentiation of amoebic and bacillary dysentery. Diagnosis. 1-Parasitic diagnosis a-Intestinal amoebiasis - stool -rectal ( exudate ) swab -material collected from the base of rectal ulcers b-Amoebic liver abscess -aspirated pus. A-Stool

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diagnosis
Diagnosis

1-Parasitic diagnosis

a-Intestinal amoebiasis

-stool

-rectal (exudate) swab

-material collected from the base of rectal ulcers

b-Amoebic liver abscess

-aspirated pus

slide5

A-Stool

1)Microscopy

-wet preparation (general stool examination)

a-saline wet preparation (trophozoites)

b-iodine wet preparation (cyst)

-fixed preparation (permanent staining)

-iron haematoxylin

-trichrome

2) Concentration

3) Culture

slide6

2-Serodiagnosis

1)Antibody Detection

-IHA

-IFAT

-ELISA

2) Antigen Detection

3-Molecular diagnosis

PCR (polymerase chain reaction)

4-Radio-imaging diagnosis

US, CT scan, MRI

epidemiology
Epidemiology

-Geographical distribution

worldwide distribution

10 percent of the world's population

100,000 persons die

-Source of transmission and infection

Food and water

cysts carriers

-Infective form

quadri-nucleated cyst

-Susceptible population

All ages

treatment
Treatment

1-Luminal amoebicides

diloxanidefluorate

2-Tissue amoebicides

metronidazole (flagyl) {submucosa and liver tissue}

chloroquine {liver tissue}

tetracycline {submucosa}

blastocystis hominis
Blastocystishominis
  • -Morphological forms: vacuolated, amoeboid, granular and cyst forms.
  • -Distribution: worldwide.
  • -Habitat: large intestine.
  • -Infective stage: cyst form.
  • -Pathogenic role: is doubtful.
  • -Diagnostic specimen: stool.