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THE OPTICAL ABSORPTION SPECTRA OF Fe 2+ and Fe 3+ IONS IN THE BLOOD. Maksims Poļakovs Institute of Solid State Physics, University of Latvia, Kengaraga street 8, LV-1063 Riga, Latvia. The aims of work. Geometrical parameters of erythrocytes
Institute of Solid State Physics, University of Latvia, Kengaraga street 8,
LV-1063 Riga, Latvia
Venous blood was donated by consenting Chernobyl clean-up workersand healthy men. Blood was collected under air in glass tubes containing a small amount of heparinused as an anticoagulant. Blood without any anticoagulant wasalso testedand showed no difference with respect heparinblood.
Clinical investigations show high morbidity rate of clean-up workers compare with general population.
Nowadays they represent group of chronically sick people with diseases prevalence of digestive, musculoskeletal and nervous system.
Most of the Chernobyl’ clean-up workers have poli-symptomatic sicknesses that exhibit tendency to progress.
The diameter of a typical human erythrocyte disk is 6–8 µm, much smaller than most other human cells. A typical erythrocyte contains about 270 million hemoglobin molecules, with each carrying four heme groups.
Adult humans have roughly 2–3 × 1013 red blood cells at any given time (women have about 4 million to 5 million erythrocytes per cubic millimeter (microliter) of blood and men about 5 million to 6 million; people living at high altitudes with low oxygen tension will have more). Red blood cells are thus much more common than the other blood particles
AFM image of erythrocytes
P - Perimeter of RBCS - Square of RBCf - Form factorof RBC (P2/S)
D - The maximal size of RBCW - The minimal size of RBCExt - Eccentricity of RBC ( D/W )
Slim - parametrs of erytrocytes for Chernobyl`s clean-up wokers
Norma – parametrs of erytrocytes for practically heath peopele
the heme ironis 6-coordinated
Ion of iron Fe2+ is bounding up with molecule of O2 does not change valence, but transferring from high to low spin state.
Dq high spin < Dq low spin
Low spin S=0
High spin S=2
Fe(II)-heme is oxidized to Fe(III)-heme to form metHb.
MetHb does not bind O2.
Dq high spin < Dg low spin
Low spin S=1/2
High spin S=5/2
Absorption spectrum of Methemoglobin and Hemoglobin
We observed that geometrical parameters of erythrocytes for healthy people differ from geometrical parameters for sick people.
We observed the additional absorption bands 630-660 nm in absorption spectra of blood of Chernobyl clean–up worker who has erythrocytosis. These data shows that the blood of the Chernobyl`s clean –up workers which have erythrocytosis met hemoglobin higher
Perhaps the ion Fe 2+ in the-heme of hemoglobin is oxidized to the Ion Fe3+ in the heme by radiation. While estimating the health status of clean-up workers part of the revealed pathology could be explained by the higher levels of strontium and other radionucleids in the body.