the optical absorption spectra of fe 2 and fe 3 ions in the blood n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
THE OPTICAL ABSORPTION SPECTRA OF Fe­ 2+ and Fe­ 3+ IONS IN THE BLOOD PowerPoint Presentation
Download Presentation
THE OPTICAL ABSORPTION SPECTRA OF Fe­ 2+ and Fe­ 3+ IONS IN THE BLOOD

Loading in 2 Seconds...

play fullscreen
1 / 19

THE OPTICAL ABSORPTION SPECTRA OF Fe­ 2+ and Fe­ 3+ IONS IN THE BLOOD - PowerPoint PPT Presentation


  • 98 Views
  • Uploaded on

THE OPTICAL ABSORPTION SPECTRA OF Fe­ 2+ and Fe­ 3+ IONS IN THE BLOOD. Maksims Poļakovs Institute of Solid State Physics, University of Latvia, Kengaraga street 8, LV-1063 Riga, Latvia. The aims of work. Geometrical parameters of erythrocytes

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'THE OPTICAL ABSORPTION SPECTRA OF Fe­ 2+ and Fe­ 3+ IONS IN THE BLOOD' - marah-casey


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
the optical absorption spectra of fe 2 and fe 3 ions in the blood

THE OPTICAL ABSORPTION SPECTRA OF Fe­2+ and Fe­3+ IONS IN THE BLOOD

Maksims Poļakovs

Institute of Solid State Physics, University of Latvia, Kengaraga street 8,

LV-1063 Riga, Latvia

slide2

The aims of work

  • Geometrical parameters of erythrocytes
  • In the present work we report results of measurements optical absorption of blood.
used equipments
Used equipments
  • For optical absorption spectra measurements was used “Specord UV-VIS”.
  • For geometrical parameters of erythrocytes was used Automatic analyzer of microscopic image “MORPHQUANT”
materials
Materials

Venous blood was donated by consenting Chernobyl clean-up workersand healthy men. Blood was collected under air in glass tubes containing a small amount of heparinused as an anticoagulant. Blood without any anticoagulant wasalso testedand showed no difference with respect heparinblood.

slide5

Clinical investigations show high morbidity rate of clean-up workers compare with general population.

Nowadays they represent group of chronically sick people with diseases prevalence of digestive, musculoskeletal and nervous system.

Most of the Chernobyl’ clean-up workers have poli-symptomatic sicknesses that exhibit tendency to progress.

slide6
Some of clean-up workers have erythrocytosis.Erythrocytosis is defined as an excess of erythrocytes, or red blood cells (RBCs).RBCs in the blood are measured by the hematocrit (the percentage of the blood volume made up of RBCs) or by the hemoglobin
slide7

erythrocytes

The diameter of a typical human erythrocyte disk is 6–8 µm, much smaller than most other human cells. A typical erythrocyte contains about 270 million hemoglobin molecules, with each carrying four heme groups.

Adult humans have roughly 2–3 × 1013 red blood cells at any given time (women have about 4 million to 5 million erythrocytes per cubic millimeter (microliter) of blood and men about 5 million to 6 million; people living at high altitudes with low oxygen tension will have more). Red blood cells are thus much more common than the other blood particles

AFM image of erythrocytes

slide8

Geometrical parametrs of erythrocytes (RBC)

P - Perimeter of RBCS - Square of RBCf - Form factorof RBC (P2/S)

D - The maximal size of RBCW - The minimal size of RBCExt - Eccentricity of RBC ( D/W )

slide9

Result:

Slim - parametrs of erytrocytes for Chernobyl`s clean-up wokers

Norma – parametrs of erytrocytes for practically heath peopele

slide10

Hemoglobin structure

  • Hemoglobin is nanoparticlewith sizes 6,4 x 5,5 x 5,0 nm
  • Composed of four globular protein subunits (tetramer) each with a heme group.
  • Four types of globin chains occur in pairs containing 141-146 amino acids
  • Responsible for cells ability to transport O2 and CO2
  • When a ligandis bound to Hb,

the heme ironis 6-coordinated

slide11

Heme structure of Hemoglobin

Ion of iron Fe2+ is bounding up with molecule of O2 does not change valence, but transferring from high to low spin state.

slide12

Hemoglobin Fe 2+ low and high spin state

Fe2+ [Ar]3d6

Dq high spin < Dq low spin

eg

eg

t2g

t2g

Low spin S=0

(oxyhemoglobin)

High spin S=2

(deoxyhemoglobin)

slide14

Methemoglobin

Fe(II)-heme is oxidized to Fe(III)-heme to form metHb.

MetHb does not bind O2.

oxy-Hb met-Hb

slide15

oxyHb(Fe2+)

metHb(Fe3+)

slide16

Methemoglobin Fe 3+ low and high spin state

Fe3+ [Ar]3d5

Dq high spin < Dg low spin

eg

eg

t2g

t2g

Low spin S=1/2

High spin S=5/2

slide18

Optical absorption spectra of oxy-hemoglobin (Fe2+ ; S=0)

Absorption spectrum of Methemoglobin and Hemoglobin

slide19

CONCLUSIONS

We observed that geometrical parameters of erythrocytes for healthy people differ from geometrical parameters for sick people.

We observed the additional absorption bands 630-660 nm in absorption spectra of blood of Chernobyl clean–up worker who has erythrocytosis. These data shows that the blood of the Chernobyl`s clean –up workers which have erythrocytosis met hemoglobin higher

normal.

Perhaps the ion Fe 2+ in the-heme of hemoglobin is oxidized to the Ion Fe3+ in the heme by radiation. While estimating the health status of clean-up workers part of the revealed pathology could be explained by the higher levels of strontium and other radionucleids in the body.