Nutrition, Growth, and Metabolism. In Medical Microbiology, microbial cultivation is required for the following purposes In Medicine 1-Diagnosis of most infectious diseases 2-Selection of drug of choice ( antibiotics ) for treatment of infection In other fields
In Medical Microbiology, microbial cultivation is required for the following purposes
During stationary phase, the growth rate slows as a result of nutrient depletion and accumulation of toxic products
In the absence of β-lactam antibiotics, the bacterial cell wall plays an important role in bacterial reproduction.
Adding β-lactam antibiotics to the cell medium while bacteria are dividing will cause them to shed their cell walls and fail to divide, forming large, fragile spheroplasts.