ABSTRACT. The in vitro toxicity test of water phase from ethanol extract of Justicia. gendarussa Burm.f leaves to human normal lymphocyte cell. According to previous research on antifertility activity of Justicia
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
The in vitro toxicity test of water phase from ethanol extract of Justicia
gendarussa Burm.f leaves to human normal lymphocyte cell
According to previous research on antifertility activity of Justicia
gendarussa Burm.f, it was found to be potentially developed as herbal medicine.
Hence, it must be followed by premedical research then continued to clinical trials.
One of the premedical research is toxicity test. Its need to be done to know the safety
This research was intended to find toxicity effect of water phase from ethanol
extract of Justicia gendarussa Burm.f leaves on lymphocyte cells culture by addition
of MTT (3-(4,5-dimethilthiazol-2-yl)-2,5-diphenyl tetrazolium bromide). The
consentration of test samples are 126 ppm, 252 ppm, 504 ppm, and 1008 ppm.
2x105 cells/mL suspension of lymphocyte cells were added in 96 tissue
culture clusters. The water phase of Justicia gendarussa Burm.f leaves were added to
the wells with that consentration in triplicate, then incubated for 24 hours. Following
the addition of MTT, the cell was incubated further for four hours, and then the
reaction was stopped by addition of SDS, and measured the optical density (OD) at
wave length 550 nm using ELISA reader. The dead cells were then determined based
on the OD. The result showed that water phase from ethanol extract of Justicia
gendarussa Burm.f leaves have LC50 value 3215,7242 ppm.
Keywords : toxicity, Justicia gendarussa Burm.f, lymphocyte cell