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Field of View of a Microscope. Specimens are measured in microns (µm)! 1 millimeter = 1000 microns (micro meters). If this caterpillar is 20mm long, how many microns is it?. (20mm)(1000) = 20,000 microns. Which magnification will show more space, 40X or 100X?.

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Presentation Transcript
slide2

Specimens are measured in microns (µm)!

1 millimeter = 1000 microns (micro meters)

If this caterpillar is 20mm long, how many microns is it?

(20mm)(1000) = 20,000 microns

slide4

Ruler at 40X

How many mm across?

3mm

How many microns across?

3,000 µm

3 X 1000 = 3,000 µm across

ruler at 100x
Ruler at 100X
  • How many millimeters? Microns?
use the equation on your lab sheet to calculate the field of view at 400x
Use the equation on your lab sheet to calculate the field of view at 400X

High power magnification

Low power magnification

Low power diameter

High power diameter

=

slide7

400

40

3 mm

X

(40)(3mm) = (400)(X)

=

120mm = 400X

120mm

400

0.3mm

=

at 400x you only see 0 3 mm across
At 400X you only see 0.3 mm across

How many microns is that?

0.3mm X 1000 = ?

300 µm

estimating the size of objects
Estimating the size of objects

If the field of view is 600 microns across, how far across is one specimen?

how many specimen can fit across
How many specimen can fit across?

If you can fit 6 across 600 microns, how far across is one circle?

Diameter / # of objects

600 microns/6 circles = 100 microns each

slide12

40X

100X

400X

slide13

40X

100X

400X