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RDP vs BCR-P vs SDP DDr.SusheelaInnah Professor &HoD Dept of Transfusion Medicine Jubilee Mission Medical College, Thrissur
History • In 1910 Duke first noted that platelets from whole blood reduce the bleeding time • In 1950s platelets were collected and transfused, but only for diagnostic purposes. • In 1960s plastic blood bags became available which enabled platelets to be separated from whole blood collections by centrifugation.
In the 1970s Drs. Scott Murphy and Frank Gardner discovered that platelet function was best preserved if the platelets were stored at room temperature with agitation. This resulted in the introduction of prophylactic platelet transfusions for patients with leukaemia. • In the 1980s new methods for platelet transfusion were introduced using cytapheresis technique • In the 1990s cytapheresis methods was refined by collecting leucocyte reduced platelet products.
Platelet Products Available for Transfusion Platelets are obtained by three different methods: • Platelet concentrates from whole blood by hard spin (Random donor platelets) - RDP • Buffy coat removed platelet concentrate – BCR-P • Apheresis Platelets (Single Donor Platelets) - SDP
Preparation of platelet concentrate Plasma RBCs PRP Platelet concentrate
RDP • The hard-spinning of the platelets on to the bottom of the bag in the PRP method, requiring re-suspension of the plts, may induce a collection injury that could potentially compromise the long-term storageof PRP platelets compared with BC platelets. However, there is currently no direct evidence to substantiate this hypothesis Transfusion 1989;29:605-609 Blood 2003;102:93a. Blood 2006;108:282a-283a.
Buffy Coat Removed Platelets Buffy coat removed cells prepared by centrifugation and separation by automated Component separator
Preparation of leuco-reduced components using top and bottom bags with optipress PPP WB BC BCR-RBC
Buffy coat PPP BCR-RBC PPP BC BC BC BCR-RBC
Leuco-reduced platelets BC-PC BC-PC BCR BC BCR
Sterile connecting device for preparing pooled platelets BC BC BC BC BC BCP
Single Donor Platelets • Single-donor apheresis platelet concentrates may be collected by a variety of apheresis systems, using different protocols. Platelet yields may vary, and each procedure or protocol must be fully validated, documented and specifications set.
AUSTRALASIAN SOCIETY OF BLOOD TRANSFUSION Topics in Transfusion Medicine February 2001 Vol 8 No 1
Conclusion • Thus administering random donor platelets followed by single donor platelets if alloimmunizationdevelops, is justified by both economic and scientific reasoning.