summary of electron transport l.
Skip this Video
Loading SlideShow in 5 Seconds..
Summary of electron transport PowerPoint Presentation
Download Presentation
Summary of electron transport

Loading in 2 Seconds...

play fullscreen
1 / 31

Summary of electron transport - PowerPoint PPT Presentation

  • Uploaded on

Summary of electron transport. There can be branches, at terminal electron acceptor, at terminal oxidase, at entry point of NADH (ie. hot stinking plants). Unfortunately, oxygen is not just a terminal electron acceptor. NADH, a great source of energy.

I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
Download Presentation

PowerPoint Slideshow about 'Summary of electron transport' - lot

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.

- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
summary of electron transport
Summary of electron transport
  • There can be branches, at terminal electron acceptor, at terminal oxidase, at entry point of NADH (ie. hot stinking plants)
nadh a great source of energy
NADH, a great source of energy
  • NADH + 11 H+ + ½ O2 NAD+ + 10 H+ + H2O
  • Highly exergonic; DGo = -220 kJ/mol
  • Actually in cell, much NADH than NAD, making the available free energy more negative
  • Much of this energy is used to pump protons out of the matrix
generation of a proton motive force
Generation of a proton-motive force
  • In an actively respiring mitochondria, the pH is ~0.75 units lower outside than in the matrix
  • Also generates an electrical potential of 0.15 V across the membrane, because of the net movement of positively charged protons outward across the membrane (separation of charge of a proton without a counterion)
  • The pH difference and electrical potential both contribute to a proton motive force

Really, what does that mean?

  • Energy from electron transport drives an active transport system, which pumps protons across a membrane. This action generates an electrochemical gradient through charge separation, and results in a lower pH outside rather than in. Protons have a tendency to flow back in to equalize the pH and charge. This flow is coupled to ATP synthesis.
measuring the proton motive force
Measuring the proton motive force

DmH = Dy – 2.3RTDpH/F

(different in Lehninger)

mH is the resulting proton motive force (sometimes p)

y is the electrochemical membrane potential

pH has a negative value, thus contribution is positive in this equation

don t get bogged down in the math but
Don’t get bogged down in the math, but …
  • (under standard conditions) DmH = 0.224 V

Plug into DGo = -nFDEo and DGo = ~20 kJ/mole H+

The bottomline 

the proton motive force bottom line
The proton motive force bottom line

Two components to energy derived from electron transport, pH and electrical potential. The electrical potential is the primary contributor to free energy.

Most of the energy from oxidation of NADH is conserved in the proton gradient

electron transfer and atp synthesis are coupled
Electron transfer and ATP synthesis are coupled
  • ATP synthesis occurs only if electron transfer does, and vice-versa
  • When isolated mitochondria are suspended in buffer containing ADP, Pi and an oxidizable substrate (succinate) three things happen
    • Substrate is oxidized
    • Oxygen is consumed
    • ATP is synthesized
all components are essential
All components are essential
  • If ADP were omitted, no ATP synthesis would occur and electron transfer to oxygen does not proceed, as well.

Black – oxygen consumption

Red – ATP synthesis

there are compounds that can inhibit atp synthesis
There are compounds that can inhibit ATP synthesis
  • The antibiotic oligomycin binds to ATP synthase and inhibit it’s action.
  • By stopping ATP synthesis, this compound also stops electron transport.
  • Because oligomycin is specific for ATP synthase and not the various electron carriers, this inhibition supports the coupling of ATP synthesis to electron transport
there are compounds that can uncouple atp synthesis from electron transport
There are compounds that can uncouple ATP synthesis from electron transport
  • DNP and FCCP block ATP synthesis, while permitting continued electron transport to oxygen – they are uncouplers
  • They pick up protons from the outside, diffuse in (they are hydrophobic so can pass through the membrane), and release proton back inside.
  • Electrons are still passed through the electron transport chain, but the proton gradient is destroyed.
something we ll cover when we talk about enzymes in detail
Something we’ll cover when we talk about enzymes in detail:
  • ATP synthase stabilizes ATP relative to ADP + Pi by binding ATP more tightly, this results in a free energy change that is near zero
  • This is an important point, but ignore for the most part now as we will cover this in detail later
  • What’s important now is that this reaction ATP synthesis from ADP and Pi occurs without a huge input of energy – you’ll see it is just mechanical energy.
atp synthase has two functional domains
ATP synthase has two functional domains
  • This enzyme has two distinct parts, one a peripheral membrane protein (F1) and one a integral membrane protein (Fo) ( the o stands for oligomycin sensitive)
  • These parts can be separated biochemically, and isolated F1 catalyses ATP hydrolysis (it has the site for ATP synthesis and hydrolysis)
the f 1 component
The F1 component
  • This component is made up of nine proteins of five different types with a composition of: a3b3gde
  • Each of the three b subunits have a catalytic or “active” site where the reaction occurs
    • ADP + Pi  ATP + H2O
conformational changes
Conformational changes
  • Although the b subunits have the exact same amino acid sequence and composition, they are in different conformations due to the g subunit.
  • These conformational differences affect how the enzyme binds ATP and ADP
rotation of the g subunit by h translocation drives atp synthesis
Rotation of the g subunit by H+ translocation drives ATP synthesis
  • Passage of protons through the Fo component causes g to rotate in that internal chamber
  • Each rotation of 120o causes g to contact another b subunit, this contact forces b to drop ATP and stay empty
  • The three b subunits interact so that when one is empty, one has ADP and Pi, while another has ATP.
atp synthase at work
ATP synthase – at work
regulation of etc
Regulation of ETC
  • Rate of mitochondrial respiration controlled by ADP availability ([ATP]/[ADP][Pi])
  • IF1 can bind and block ATP synthase at low pH
  • Hypoxia influences gene expression
what happens when
What happens when…
  • Cells increase NADH oxidation using alternative NADH oxidase?
  • Cells using lots of ATP