Nucleotide - based information - PowerPoint PPT Presentation

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Nucleotide - based information

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  1. Our Service Portfolio Nucleotide - basedinformation Transcripts : mRNA - SuperSAGE, ST-DGE - RNAseq - qRT-PCR, Taq-Man assays, Real-Time PCR service - NormalizationofcDNAlibraries (qualitative information) - Massive Analysis ofcDNA Ends (MACE) non coding RNA - MicroRNA - Degradome Genomic DNA: - Digital karyotyping (DK), copynumbervariations (CNVs) - Methylation-specific DK (MSDK) - Genotyping - Identificationof SNPs - Molecularmarkers

  2. Massive Analysis ofcDNAEnds: MACE How it works cDNA population bound to Streptavidine AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA Streptavidin-Beads 5’ 3’ AAAAAAA-3’ TTTTTTT-5’ cDNA 5’ 3’ AAAAAAA-3’ TTTTTTT-5’ cDNA AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA

  3. Massive Analysis ofcDNAEnds: MACE How it works Fragmentation AAAAAAA-3’ TTTTTTT-5’ Streptavidin-Beads AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ 100-200 bp

  4. Massive Analysis ofcDNAEnds: MACE How it works 2nd generation sequencing of 50-100 bp AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’

  5. Massive Analysis ofcDNAEnds: MACE How it works Assembly & Counting AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’

  6. Massive Analysis ofcDNAends: MACE How it works Assembly & Counting 1 1 4 Counting, BLAST 50-200bp

  7. Massive Analysis ofcDNAends: MACE SNP-Analysis SNP Rattusnorvegicus, Laboratory Strain ClustalXviewof MACE-sequences

  8. Massive Analysis ofcDNAEnds: MACE How it works Summary • Longer tags aregenerated: upto 200 bp after assembly • Similardepthofanalysis: rare transcriptsareincluded • More splicingvariantsaredetected: • random vs. restrictionenzymebased tag-position