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Histologic Quality Control Assessment of Tissue Samples Procured for Research Purposes. Procedures used by CHTN Prepared: December 2006 Updated: January 2010. BACKGROUND. The majority of CHTN tissue collection is performed in the context of excess or “leftover” tissue present

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slide1

Histologic Quality Control Assessment of Tissue Samples Procured for Research Purposes

Procedures used by CHTN

Prepared: December 2006

Updated: January 2010

slide2

BACKGROUND

  • The majority of CHTN tissue collection is performed
  • in the context of excess or “leftover” tissue present
  • in specimens resected from patients as part of their
  • routine clinical care
  • Fresh and frozen tissue samples have matched
  • tissue samples fixed in formalin and embedded in
  • paraffin for histologic sectioning
slide4

BACKGROUND (cont.)

  • Tissue samples are provided to investigators with
  • pertinent clinicopathologic data obtained from the
  • surgical pathology report and other clinical records
  • The histologic slides from the FFPE tissue are
  • examined for tissue content and agreement with
  • associated clinicopathologic data by an Anatomic
  • Pathologist
slide5

QUALITY CONTROL DATA COLLECTED

  • Anatomic site of procured tissue
  • Tissue classification:
    • Malignant neoplasm
    • Neoplasm indeterminate for malignancy
    • Benign neoplasm or mass
    • Diseased (non-neoplastic)
    • Normal
slide6

QUALITY CONTROL DATA COLLECTED (cont.)

  • If malignant neoplasm:
    • Primary
    • Recurrent
    • Metastatic
    • Uncertain if primary or metastatic
  • If metastatic, site of primary tumor
  • Additional diagnostic classification (e.g. specific disease classification from clinical pathology report)
  • If QC material consistent with annotated clinicopathologic data
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QUALITY CONTROL DATA COLLECTED (cont.)

  • If a neoplasm (tumor) is present in the tissue section, two
  • different assessments of tumor percentage are made:
    • % cellularity
    • an estimate of the number of tumor cells in the histologic section relative to
    • the total number of cells
    • % area
    • an estimate of the area of tissue involved by tumor
  • The % area and % cellularity may be discrepant if:
    • The tumor is invested by a large number of inflammatory
    • cells
    • The tumor has a high degree of fibrosis
    • The tumor has large areas of acellular mucin
slide8

QUALITY CONTROL DATA COLLECTED (cont.)

  • Tumor attributes that are assessed:
    • % tumor necrosis, by cellularity
      • (using only tumor cells as denominator)
    • % non-neoplastic stroma, by area
      • (using only tumor area as denominator)
    • % acellular mucin, by area
      • (using only tumor area as denominator)
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EXAMPLES OF HISTOLOGIC QC

  • Adenocarcinoma of the Prostate
  • Adenocarcinoma of the Colon
  • Lobular Breast Carcinoma
  • Adenocarcinoma of the Pancreas
  • Mucinous adenocarcinoma of the Colon