MII. end. entry. MI. Single Cell Informatics. Motivation. Some phenomena can only be seen when filmed at single cell level! (Here: excitability). Outline. Motivation: Similar cells respond differently most methods don’t see that: uarrays, gels, blots Possible reasons:
Some phenomena can only be seen when filmed at single cell level! (Here: excitability)
sporulationDecision making in cells:switching from one state to another
cell state change
Similar cells respond differently to the same signal
What can lead to variable responses?
The cells differ in some aspects (type, size, …)
Difference between cells: time of decision
meiosis & sporulation
We can fluorescently tag different levels along this pathway!
These have to be tailored to cell type, motility, signal location, etc.
11.1±2.2hrTwo-color use for event annotation
Adding another fluorescent marker allows annotating more events.
Hypothesis: meiosis entry is determined by last mitosis
Htb2-mCherry ▄▄Dmc1-YFP ▄▄
Conclusion: Countdown to meiosis occurs in parallel to the cell cycle
cell cycle phase
onset time of early genes
Large number of single cell measurements let us build a model of causative links between molecular levels, phenotypes, event timings.
The time tracks verify the circuit model:
The red and green genes are anti-correlated