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Things to Do Today

Things to Do Today. Observe Urinalysis Plates Note hemolysis & Count Colonies on BAP Observe EMB plate Blood Lecture Blood Typing Differential WBC Count Observe Parasitic Blood Slides. Whole Blood. Plasma Buffy coat Hematocrit. RBCs. RBC Disorders. Anemias -hemolytic

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Things to Do Today

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  1. Things to Do Today • Observe Urinalysis Plates • Note hemolysis & Count Colonies on BAP • Observe EMB plate • Blood Lecture • Blood Typing • Differential WBC Count • Observe Parasitic Blood Slides

  2. Whole Blood • Plasma • Buffy coat • Hematocrit

  3. RBCs

  4. RBC Disorders • Anemias -hemolytic -Iron deficiency -aplastic -sickle cell Polycythemia

  5. WBCs Wikipedia Commons, used with permission

  6. WBCs neutrophil eosinophil basophil monocyte lymphocyte Wiley

  7. WBCs WebPath, used with permission

  8. WBCs Vs. lymphocyte monocyte Medicine USC, used with permission

  9. Leukocyte Disorders • Leukemia • Infectious mononucleosis • Leukopenia

  10. Formation of platelets

  11. Clotting

  12. Blood Types

  13. Hemolytic DZ of the Newborn • Mom must be Rh (-) • Father must be Rh (+) • A child must be Rh (+) • A subsequent child must be Rh (+)

  14. Hemolytic DZ of the Newborn

  15. Blood typing

  16. Watch demo of Blood Smear 10 sec in dye 20-24 sec in rinse

  17. Blood Parasites Microbe Disease • Trypanosoma cruzi Chaga’s disease • Leishmania Leishmaniasis • Plasmodium vivax Malaria • Trypanosoma gambiense Sleeping sickness

  18. Trypanosoma cruzi 1000x Etiological Agent for Chaga’s disease KM

  19. Rhodnius: Vector for Chaga’s disease AKA assassin bug; kissing bug KM

  20. Blood Parasites Microbe Disease • Trypanosoma cruzi Chaga’s disease • Leishmania Leishmaniasis • Plasmodium vivax Malaria • Trypanosoma gambiense Sleeping sickness

  21. Leishmania Life Cycle

  22. Leishmania Vector: sandfly 1000x KM

  23. A Patient Suffering from leishmaniasis

  24. leishmaniasis DermAtlas, used with permission

  25. leishmaniasis DermAtlas, used with permission

  26. leishmaniasis DermAtlas, used with permission

  27. leishmaniasis Dermis.net, used with permission

  28. leishmaniasis Dermatology Image Atlas, used with permission

  29. Blood Parasites Microbe Disease • Trypanosoma cruzi Chaga’s disease • Leishmania Leishmaniasis • Plasmodium vivax Malaria • Trypanosoma gambiense Sleeping sickness

  30. Malaria • Causative agent: Protozoan Plasmodium • One of the most severe of all parasitic diseases • Endemic is most tropical areas • Plasmodium are amoeboid, intracellular protozoa that infect erythrocytes and other tissues • Transmitted by the female Anopheles mosquito

  31. The Worldwide Incidence of Malaria

  32. The life cycle of the malaria parasite Plasmodium

  33. Malaria in RBCs The “ring” stage of the malarial parasite Plasmodium falciparum Seen as dark, circular structures within red blood cells

  34. Plasmodium vivax Malaria 1000x KM

  35. Blood Parasites Microbe Disease • Trypanosoma cruzi Chaga’s disease • Leishmania Leishmaniasis • Plasmodium vivax Malaria • Trypanosoma gambiense Sleeping sickness

  36. Trypanosoma gambiense 1000x Sleeping Sickness Vector: Tsetse fly KM

  37. Blood Types Type A Type B Type AB Type O Anti A antibody Anti B antibody

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