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Things to Do Today

Things to Do Today. Exam & Go over Exam. Throat Cultures Unknown gm (-) rod ID Assign HW: Fill in Table 1. Gram + ID. BAP Hemolytic Reactions. Used with permission. BAP Hemolytic Reactions. Alpha hemolysis. Beta hemolysis. gamma. KM. Perform throat Swab. 1 BAP per person

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Things to Do Today

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  1. Things to Do Today • Exam & Go over Exam. • Throat Cultures • Unknown gm (-) rod ID • Assign HW: Fill in Table 1. Gram + ID

  2. BAP Hemolytic Reactions Used with permission

  3. BAP Hemolytic Reactions Alpha hemolysis Beta hemolysis gamma KM

  4. Perform throat Swab 1 BAP per person Swab first Quadrant and then smear swab on slide (throat) Use loop, flaming in between quadrants, and finish the 4 quadrant streak. Heat fix the slide throat plate

  5. Gram (-) Test results Organism oxidase Urease MR H2S Gas Glucose Lactose Citrate Escherichia - - + - + + + - Pseudomonas + - - - - - - + Morganella - + - - + - - Proteus - + + + + + - - Enterobacter - - - - + + + + Salmonella - - - + + + - + Providencia - + - + + - - Unknown microbe

  6. Oxidase Test Positive Negative

  7. Urease Test Negative Positive KM

  8. Methyl Red Test Negative Positive

  9. KIA Slants uninoculated

  10. KIA Slants uninoculated KM

  11. Citrate test Negative positive KM

  12. Gram Negative Unknown • Obtain unknown and write down number in your notes and on ALL media • Inoculate the MR and Urease broth tubes • Inoculate the Citrate agar by streaking its surface • Inoculate the KIA by streaking its surface AND then stabbing the butt.

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