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DOSAGE DU GLUCOSE

DOSAGE DU GLUCOSE. Par spectrophotométrie. BUT DU TP. Doser le glucose contenu dans une solution Par un dosage spectrophotométrique En utilisant les propriétés réductrices du glucose . A =   x C x l. PRINCIPE DU TP.

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DOSAGE DU GLUCOSE

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  1. DOSAGE DU GLUCOSE Par spectrophotométrie

  2. BUT DU TP • Doser le glucose contenu dans une solution • Par un dosage spectrophotométrique • En utilisant les propriétés réductrices du glucose

  3. A =  x C x l PRINCIPE DU TP • Réduction de l’acide picrique (jaune) en acide picramique (brun) par le glucose ACIDE PICRIQUE PRODUITS D’OXYDATION ACIDE PICRAMIQUE GLUCOSE + +  = 570 nm

  4. MODE OPERATOIRE

  5. RESULTATS GAMME ETALON D’ACIDE PICRIQUE SOLUTION A DOSER

  6. RESULTATS

  7. GAMME D’ETALONNAGE 2,45 mg

  8. CALCUL DE LA CONCENTRATION • Détermination de la concentration de la solution X • Données : • Mglucose = 2,45 mg = 2,45.10-3g • V = 2 mL = 2.10-3 mL • Calcul  = m/V = 2,45.10-3/2.10-3 = 1,23 g.L-1

  9. CONCLUSION • CONCENTRATION DE LA SOLUTION A DOSER EST DE 1,23 g.L-1

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