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Welcome

Welcome. What is Engineering World Health at UCSD about? . Providing real world, project-based experience focused on medical technology Partnering with EWH national to help individuals and hospitals in developing countries

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Welcome

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  1. Welcome

  2. What is Engineering World Health at UCSD about? • Providing real world, project-based experience focused on medical technology • Partnering with EWH national to help individuals and hospitals in developing countries • Educating engineering students on the growing efforts by UCSD Faculty in both Medicine in Engineering to improve Global Health We are committed to:

  3. Upcoming Events • Global Health Speaker Series • Wednesday, Jan. 25 at 6:30pm, Fung Auditorium • Dr. Richard Garfein • Professor of Global Health • Extensive overseas experience in infectious disease • Electrosurgery Tester Kit Build Day • Saturday, Feb. 11 in PFBH teaching lab • Team-based activity • Summer Program in Tanzania • Project Team Meetings

  4. Slide about next week’s speaker • Dr. Richard Garfein • Mobile technology in treatment of tuberculosis • Wednesday, January 25 at 6:30pm (exactly one week from now) • Fung Auditorium

  5. Build Day – Feb. 11 • Hands on soldering and project experience • Assemble electrosurgery tester kits from EWH National • Kits used in low resource settings to diagnose malfunctioning equipment • $5 to help cover costs • $20 for us to order

  6. Duke Summer Program • EWH Summer Institute • Tanzania and Central America • One month training, one month work experience in developing hospitals • Learn the language, take technical classes, get lab experience, work in local hospitals • Applications due January 28 • http://ewh.org/index.php/programs/institutes

  7. RNA Extraction Team Jessica Van Allen

  8. Process • Lyse blood serum • Remove components which hinder PCR • Remove salts, proteins, and other cellular impurities

  9. Challenges • Take a process with significant user input and make it almost completely automated • No contamination • Durable • Easy to Use • Inexpensive

  10. Design • Fluid metering system replaces pipettes • Fluid flow system replaces centrifuges • Areas of focus for the quarter: • Pressure control system • Valve configuration • Electronic timing • Tubing

  11. Quarter Goals • Design a pressure control method • Identify valve dimensions and configuration • Identify material and dimensions for tubing • Link system to electronic control

  12. Thermocycler Team Justin Wong, Alexander Lin

  13. Process • Obtain Sample from RNA team • Heat Sample & Cool Sample according to protocol • Export product to Gel team

  14. Challenges • Temperature uniformity • Ramp rate (Heating/Cooling everything quickly) • Contamination • Cost • Durability/Repeatability

  15. Design

  16. Quarter Goals • TEC (thermoelectric cooler) chip optimization • Rough working prototype

  17. Gel Electrophoresis Team Kathryn Gent

  18. Gel Electrophoresis Objectives • Qualitatively determine the presence of HIV in the blood • Based on open source designs, build a functioning gel box for electrophoresis

  19. Process • Collect samples from Thermocycler team • Load samples into agarose gel • Run gel using electrophoresis • Analyze gel using DNA dye

  20. Challenges • Building a gel box that will accurately run the gel, but less expensively than a pre-made box • Safer alternatives to ethidium bromide • Determining ideal power source based on electricity standards

  21. Meet The Advisors • Faculty co-advisor: Dr. Melissa Micou • Faculty co-advisor: Dr. Juan Lasheras • Get to know us!

  22. Thank You • ewh.ucsd.edu • Click “Contact Us” to join the mailing list! • Click “Our Projects” to join the project team! • Feel free to email us: tchew@ucsd.edu ploury@ucsd.edu • If you’d like to join one of our project teams or have more questions, stay after and talk with us

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