Lab 6: Saliva Practical. Beer-Lambert Law. This session…. . Overview of the practical… Statistical analysis…. Take a look at an example control chart…. The Practical. Determine the thiocyanate (SCN - ) in a sample of your saliva using a colourimetric method of analysis
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Non numerical i.e what is present?
Numerical: i.e. How much is present?
Beers Law states that absorbance is proportional to concentration over a certain concentration range
A = cl
A = absorbance
= molar extinction coefficient (M-1 cm-1 or mol-1 Lcm-1)
c = concentration (M or mol L-1)
l = path length (cm) (width of cuvette)
Unknowns should ideally fall within the standard rangeBeer-Lambert Law
The mean provides us with a typical value which is representative of a distribution
Mean= the sum (å) of all the observations
the number (N) of observations
In forensic analysis, the rule of thumb is:
If n > 15 use
If n < 15 use s
1 = 68%
2 = 95%
2.5 = 98%
3 = 99.7%