chapter 2 n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Chapter 2 PowerPoint Presentation
Download Presentation
Chapter 2

Loading in 2 Seconds...

play fullscreen
1 / 9

Chapter 2 - PowerPoint PPT Presentation


  • 88 Views
  • Uploaded on

Chapter 2. DNA Biology Review. ©2002 Academic Press. chromosome. cell nucleus. Double stranded DNA molecule. Target Region for PCR. Individual nucleotides. DNA in the Cell. ©2002 Academic Press. Phosphate. Base. Sugar. Basic Components of Nucleic Acids. 5’end | Phosphate |

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Chapter 2' - kimama


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
chapter 2

Chapter 2

DNA Biology Review

©2002 Academic Press

dna in the cell

chromosome

cell nucleus

Double stranded DNA molecule

Target Region for PCR

Individual nucleotides

DNA in the Cell

©2002 Academic Press

basic components of nucleic acids

Phosphate

Base

Sugar

Basic Components of Nucleic Acids

5’end

|

Phosphate

|

Sugar—Base…

|

Phosphate

|

Sugar—Base…

|

3’end

©2002 Academic Press

base pairing of dna strands

Hydrogen bonds

5’

3’

5’

A = T

3’

A = T

G  C

hybridized

strands

G  C

A = T

T = A

Phosphate-sugar backbone

T = A

C  G

C  G

A = T

A

T

T = A

A

T

G

C

C  G

C  G

G

C

denatured

strands

C

G

5’

3’

G  C

3’

5’

Base Pairing of DNA Strands

©2002 Academic Press

basic chromosome structure

Chromosome 12

telomere

p

(short arm)

12p3

Band 3

centromere

q

(long arm)

12q5

Band 5

telomere

Basic Chromosome Structure

©2002 Academic Press

human genome contains 23 pairs of chromosomes

http://www.ncbi.nlm.nih.gov/genome/guide/

1 2 3 4 5 6 7 8 9 10 11 12

13 14 15 16 17 18 19 20 21 22 X Y

Sex-chromosomes

Human Genome Contains 23 Pairs of Chromosomes

©2002 Academic Press

types of dna markers
TYPES OF DNA MARKERS

(GATA)(GATA)(GATA)(GATA)

  • Length Variation
    • short tandem repeats (STRs)
      • microsatellites
      • simple sequence repeats (SSRs)
    • minisatellites (VNTRs)
  • Sequence Variation
    • single nucleotide polymorphisms (SNPs)
    • insertions/deletions

(CA)(CA)(CA)(CA(CA)(CA)

(G/A)

©2002 Academic Press

family inheritance of str alleles d13s317
Family Inheritance of STR Alleles (D13S317)

PCR product size (bp)

11

14

Father

12

14

Child #1

8

14

Child #2

11

12

Child #3

8

12

Mother

©2002 AP, Elsevier

comparison of rflp and pcr

Time required to obtain results

6-8 weeks with radioactive probes; ~1 week with chemiluminescent probes

1-2 days

Characteristic

RFLP Methods

PCR Methods

Amount of DNA needed

50-500 ng

0.1-1 ng

Condition of DNA needed

high molecular weight, intact DNA

may be highly degraded

Capable of handling sample mixtures

Yes (single locus probes)

Yes

Allele identification

Binning required

Discrete alleles obtained

Power of Discrimination

~1 in 1 billion with 6 loci

~1 in 1 billion with 8-13 loci (requires more loci)

©2002 AP, Elsevier

Comparison of RFLP and PCR