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Tracing Neuronal Cell Development and Maturation in the Mouse Spinal Cord

Tracing Neuronal Cell Development and Maturation in the Mouse Spinal Cord. Kelly Probst Dr. Michael Gross Laboratory. Sensory Information. Somatosensory Visual Auditory Olfactory Gustatory Vestibular. Exteroceptive Touch, Temperature, Pain Proprioceptive Body Position Interoceptive

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Tracing Neuronal Cell Development and Maturation in the Mouse Spinal Cord

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  1. Tracing Neuronal Cell Development and Maturation in the Mouse Spinal Cord Kelly Probst Dr. Michael Gross Laboratory

  2. Sensory Information • Somatosensory • Visual • Auditory • Olfactory • Gustatory • Vestibular

  3. Exteroceptive Touch, Temperature, Pain Proprioceptive Body Position Interoceptive Status of Viscera Somatosensory Information

  4. Body Brain Sensory Information Flow Visceral Afferents Spinal Cord

  5. Spinal Cord Innervation by Sensory Neurons Dorsal Root Ganglion (DRG)) Spinal Cord A A

  6. Sensory Neuron Thalamus Cerebellum Hindbrain Relay Interneuron Spinal Relay Interneurons Synaptic Connection

  7. G0 PM G1 M S G2 Neurons Born (E10.5 to E13.5) Mantle Zone Ventricular Zone

  8. Foxd3 = I2 Isl1-2 = I3 VZ Lbx1 = I4 MZ Early Embryonic Interneuron Populations

  9. ? Project Objective • Match adult ascending tracts with embryonic neural tube populations • Identify when in development these relay axonal projections reach the brain

  10. Backfill Analysis Flour-Dextran Contralateral Ipsilateral

  11. Embryo Dissection Hindbrain C1 (Atlas) Cervical Vertebrae

  12. Fill TMR-dextran Fill Filled Neuron

  13. Incubation

  14. Spinal Cord/Brain Dissection Fill Site Hindbrain-Spinal Cord Junction Cervical Region Brachial Enlargement Abdominal Region Lumbar Region and Enlargement

  15. Dorsal Section 27 (27 sections X 100 m = 2.7 mm) 1 cm Ventral E18.5 Backfilled Spinal Cord

  16. Dorsal Section 10 (10 sections X 100 m = 1.0 mm) 1 cm Ventral E17.5 Backfilled Spinal Cord

  17. Dorsal Section 28 (28 sections X 40 m = 1.1 mm) 1 cm Ventral E16.5 Backfilled Lbx1 +/- Spinal Cord

  18. Conclusions • By embryonic day 16.5, the axons of some relay interneurons have reached the hindbrain • A subset of these relay interneurons express Lbx1 and therefore are derived from the I4, I5, or I6 embryonic populations

  19. Future Goals • Backfill younger spinal cords • Backfill from specific brain regions • Co-label backfills with more marker combinations

  20. Acknowledgements Dr. Michael Gross Dr. Kevin Ahern Howard Hughes Medical Institute

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