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Recombinant DNA Bacterial Transformation Teacher Preparation Sterile Materials PowerPoint Presentation
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Recombinant DNA Bacterial Transformation Teacher Preparation Sterile Materials

Recombinant DNA Bacterial Transformation Teacher Preparation Sterile Materials

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Recombinant DNA Bacterial Transformation Teacher Preparation Sterile Materials

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  1. Recombinant DNA Bacterial TransformationTeacher Preparation Sterile Materials

  2. Recombinant Transformations • Sterilized Materials List • (per group of students) • 1 pack of 8-10 toothpicks wrapped in aluminum foil • 1 pack of 4 large paper clips bent at 90o wrapped in aluminum foil • 50 ml Sterile Calcium Chloride solution • 50 ml Sterile Water

  3. Recombinant TransformationSterile Materials(per group of students) • 1 aluminum foil packet containing 8-10 toothpicks

  4. Recombinant TransformationSterile Materials(per group of students) • 1 aluminum foil packet containing 4 large, smooth paper clips. The clips should be opened into a 90° angle and the small ends bent up.

  5. Recombinant TransformationSterile Materials • Heat the foil packages containing paper clips and toothpicks at 350oF for at least 30 minutes to sterilize.

  6. Recombinant TransformationCaCl2 Solution • Dissolve 0.75 g of CaCl2 into 50 ml of distilled water in a labeled 100-ml glass bottle with a cap.

  7. Recombinant TransformationCaCl2 Solution • Cap the bottle and place it in boiling water for 30 minutes. Cap should be "snugged" down on the bottle. • Refrigerate to prevent contamination.

  8. Recombinant Transformation Sterile Distilled Water • In a bottle with a cap, add 50 ml of distilled water. • Cap the bottle and place it in boiling water for 30 minutes. Cap should be "snugged" down on the bottle. • Refrigerate to prevent contamination.