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Bacterial Transformation With Plasmid DNA. Add plasmid* to 50 µl or 100 µl of competent cells in each of 2 tubes. * Amount of plasmid and competent cells to be determined Do not add plasmid to 50 µl or 100 µl of competent cells in each of 2 additional tubes.
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Bacterial Transformation With Plasmid DNA Add plasmid* to 50 µl or 100 µl of competent cells in each of 2 tubes. * Amount of plasmid and competent cells to be determined Do not add plasmid to 50 µl or 100 µl of competent cells in each of 2 additional tubes. Perform the following for each of the tubes of competent cells. Incubate on ice for 15 minutes. Heat shock at 42oC for 1.5 minutes. Incubate on ice for 2 minutes. Plate cells onto LB and LB/AMP plates. Incubate at 37oC overnight.
