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Real-Time PCR Analysis of VEGF mRNA Expression in Response to Chronic AII Treatment

This study investigates the real-time quantitative PCR analysis of vascular endothelial growth factor (VEGF) mRNA expression using the ABI Prism 7700 sequence detection system. RNA levels were standardized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to assess changes in expression. Primers and TaqMan probes specific for rat VEGF (NM_031836) were utilized. The effects of azelnidipine and nifedipine on VEGF mRNA expression in chronic AII-infused rats were also evaluated. Results indicate significant differences in expression levels among treatment groups, highlighting the impact of antihypertensive agents.

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Real-Time PCR Analysis of VEGF mRNA Expression in Response to Chronic AII Treatment

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  1. Real-time quantitative PCR mRNA expression of vascular endothelial growth factor (VEGF) was determined using the ABI Prism 7700 sequence detection system (Applied Biosystems, Foster City, CA, USA). The change in the expression was calculated by standardizing RNA levels corrected for glyceraldehyde-3-phosphate dehydrogenase expression in the sample. Primers and probes for VEGF (NM_031836) were as follows: rat VEGF forward primer, 5-AGAGATGAGCTTCCTGCAGCATA-3; reverse primer, 5-ACATTTACACGTCTGCGGATCTT-3; TaqMan probe, 5-FAM-CCGCTCTGAACAAGGCTCACAGTGAAC-TAMRA-3. * 400 † 300 † ‡ VEGF mRNA expression (% control) 200 100 0 Cont Nif (-) Azl AII Supplemental figure. Effects of azelnidipine or nifedipine on chronic AII-induced VEGF mRNA expression. VEGF mRNA expression was measured by quantitative real-time PCR. n=6 in each group. *P < 0.05 vs. control rats, †P < 0.05 vs. AII-infused rats, ‡P < 0.05 vs. nifedipine treated AII-infused rats.

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