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Amano Enzyme's solutions for sugar processing

Amano Enzyme's solutions for sugar processing. ~ Dextranase “Amano” L ~. Amano Enzyme China Application Development Department Qinglong Xu qinglong_xu@amano-enzyme.ne.jp. Presentation Outline. Background on D extranase L “Amano”. Trials on the use of D extranase L “Amano”.

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Amano Enzyme's solutions for sugar processing

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  1. Amano Enzyme's solutions for sugar processing ~ Dextranase “Amano” L ~ Amano Enzyme China Application Development Department Qinglong Xu qinglong_xu@amano-enzyme.ne.jp

  2. Presentation Outline Background on Dextranase L “Amano” Trials on the use of Dextranase L “Amano” Cost Evaluation

  3. Presentation Outline Background on Dextranase “Amano” L Trials on the use of Dextranase “Amano”L Cost Evaluation

  4. The average regional sugarcane output China produced 8% sugar in the whole world. 10.45 million tons (crop season 2010-2011).

  5. Current Problems Massecuites from one factory Normal crystals Mixed Crystals

  6. Current Problems The viscosity of dextran The presence of dextran in the sugar factories leads to increased viscosity, slowing of filtration, lower evaporation rates and so on.

  7. Current Problems Growth rate of sucrose crystals Furthermore, the large dextran molecule fraction (T2000) and higher concentrations of 1500 and 5000 ppm at the same supersaturation of 1.13 reduced the growth rate by 33% and 51%, respectively. E.A. Abdel-Rahman, T. Kurz. Influence of dextran concentrations and molecular fractionson the rate of sucrose crystallization in pure sucrose solutions. Journal of Food Engineering 84 (2008) 501–508.

  8. Current Problems Dextrans are responsible for crystal elongation along the C-axis! With dextran Without dextran

  9. Current Problems The loss of sugar was assumed about 7.7kg/ton sugar cause crystallization problems, and more prevalent in the caneindustry Conducive to Leuconostoc mesenteriodes growth and dextran formation Restriction ofthe application of sugar Falsely high polarization Determination error↑ Increased viscosity Sugar boiling and clarification is difficult Bad effect of acidic flocculation for beverage

  10. Current Problems Summary of to the problems associated with dextran contamination, in both the factory and the refinery.

  11. Current Problems Dextran in process streams measured at different Louisiana sugar factories(F1, F2, F3 and F4) (Cuddihy and Day, 1999) Sample from one factory in Zhanjiang, China. 1223ppm * Dextran measured by ASI2 method according to (Sarkar and Day, 1986)

  12. Basic chemical structure of dextran (α-(1→6)-α-D-glucan) (1→6)-D-glycoside (1→3)-D-glycoside branching is shown in the figure n 2 Glucose molecules are linked by (1→6)-D-glycoside bonds. 5% branching occurs in cane dextrans, mostly through (1→4), and (1→3),and to a lesser extent (1→2)-D-glycoside linkages.

  13. Principle of Dextranase Action High MW dextran (>1000 KDa) Dextranase Hydrolysis Med MW dextran (100~1000 KDa) Dextranase Lower MW dextran (45~100 KDa) Hydrolysis Dextranase Hydrolysis Oligosaccharides (2 to 10 degrees of polymerization), i.e., isomaltotriose, isomaltose Dextranase hydrolyzes(1→6)-D-Glycoside Linkages in Random “Endo”Sitesof HMW Dextran. From Eggleston et al (2005). Process. Biochem.

  14. Presentation Outline Background on Dextranase “Amano” L Trials on the use of Dextranase “Amano”L Cost Evaluation

  15. Sugar Beet Processing Dextranase Dextranase Ⅰ Extracting the juice Ⅱ Purification of the juice Dextranase Ⅲ Evaporation of the juice Ⅳ Crystallization

  16. Sugar Cane Processing Dextranase Dextranase Dextranase Ⅰ Extracting the juice Ⅱ Purification of the juice Ⅲ Evaporation of the juice Ⅳ Crystallization

  17. Use of Dextranase • Enzymatic treatment of juices • Enzymatic treatment of syrups

  18. Determination of Dextran The method in GB 15108-2006 was modified and the dextran could be examined up to 10000ppm with high accuracy. ScienceandTechnologyofFoodIndustry. In press.

  19. Effect of Dextranase Addition 10min 10min Mixed juice 0 ppm 28 ppm 56 ppm 25min 25min 60min 16hour Dextran，Amylose, Pectin… Dextran，Amylose, Pectin… Dextran，Amylose, Pectin… Dextran，Amylose, Pectin… Dextran，Amylose, Pectin… Dextran，Amylose, Pectin… Dextran was hydrolyzed to small degree of polymerization. 50,000 Da UF 4hr.→HPLC

  20. Enzymatic treatment of juices 55℃ Dextranase “Amano”L has excellent performance on the hydrolysis of dextran for mixed juice.

  21. Enzymatic treatment of juices Dextranase use levels which will normally apply for the treatment of raw juice through simulating the practical of sugar production process. Adding 10mg/kg Dextranase “Amano” L: ①dextran in clarified juice was removed 61.32%, ② settling time was cut down 13.46%, ③purity was raised 0.65%，④pH and Brix had no obvious changes.

  22. Sugar Cane Processing Enzymatic treatment of juices

  23. Enzymatic treatment of juices Combination with Microbial Fungicides

  24. Enzymatic treatment of syrups Dextranase “Amano”L is having athermal stability at up to 85 ºC and a wider pH rangebetween 3 and 7.

  25. Enzymatic treatment of syrups Dextranase “Amano” L can be used for raw sugar refining. Though it was necessary to correct the dose due to the loss ofactivity caused by the high Brix, treatment of syrup was assumed to be more effective.

  26. Presentation Outline Background on Dextranase “Amano” L Trials on the use of Dextranase “Amano”L Cost Evaluation

  27. Cost Evaluation (Income for you) Sugar cane price: 08/09, 275Υuan/ton→ 10/11年, 482 Υuan/ton ▼Dextranase Cost 1.5~2.5 Yuan/ton Sugar cane 1T 482 Yuan Dextranase 3~5ppm Mixed juice 1T Sugar processing Sugar 0.12~0.13T 840~910 Yuan Sugar price: 2009,3000 Υuan/ton→ 2011年, 7000 Υuan/ton Mixed juice Dextran: 61.32%↓ Selting time: 13.46%↓ Purity: 0.65%↑ Sugar yield: 0.5%~1％↑ Sugar 0.1212~0.1313T 848~919元 +Income 8~9Yuan Sugar 0.1212~0.1313T 852~923元 Sugar quantity: Color↓ Class 2→ Class 1 + Income 12~13Yuan

  28. Cost Evaluation (Saving for you) For example: Sugarcane 10000T /Day * Lime required to aid filtration Eggleston, G. Successful Application of DextranaseInSugar Beet Factories. 2010. • a reduction in the amount of recycle to raw juice from filter cleaning • improved factory operations • more stable beet-end flows • smoothing of vapor demands

  29. The next country? Dextranase being used

  30. Thank you for your attention! Great thanks for the help from Dr. ZG Zhang: College of Light Industry and Food Sciences, South China University of Technology, China

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