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DNA Denaturation by: -Heat -Chemicals (e.g. formamide) -High pH

Topic 2, Nucleic acids, genes, and genomes. DNA Denaturation by: -Heat -Chemicals (e.g. formamide) -High pH Melting Temperature is dependent on: -Length of DNA (for short fragments) -Base composition. Kinetics of Renaturation. *. *. highly repetitive DNA – very low C o t.

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DNA Denaturation by: -Heat -Chemicals (e.g. formamide) -High pH

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  1. Topic 2, Nucleic acids, genes, and genomes DNA Denaturation by: -Heat -Chemicals (e.g. formamide) -High pH Melting Temperature is dependent on: -Length of DNA (for short fragments) -Base composition

  2. Kinetics of Renaturation

  3. * *

  4. highly repetitive DNA – very low Cot non-repetitive DNA – higher Cot repetitive DNA – low Cot

  5. 0 2 4

  6. Yeast artificial chromosome

  7. Bacterial artificial chromosome

  8. Sequence tagged sites

  9. Mapping with STSs

  10. “Shotgun sequencing and BAC walking” Figure 24.11

  11. Physical distance versus genetic map distance

  12. What explains the conservation of gene order over large evolutionary distance?

  13. Figure 2: Neurospora possesses few highly similar genes. Figure 1: Neurospora has a low proportion of genes in multigene families.

  14. Figure 3: Example of lack of recent duplications in a Neurospora gene family (MFS sugar transporters).

  15. Figure 4: Correspondence between predicted RIP, methylation and repetitive DNA.

  16. Figure 5: Overview of major intracellular signaling pathways in Neurospora.

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