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Forensic DNA Testing. MN BCA Forensic Science Services. DNA Testing Capabilities. Short Tandem Repeats Autosomal testing Y-chromosome testing Paternal inheritance Mitochondrial sequencing Control region Maternal inheritance Databasing. Testing Results.

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forensic dna testing

Forensic DNA Testing

MN BCA Forensic Science Services

dna testing capabilities
DNA Testing Capabilities
  • Short Tandem Repeats
    • Autosomal testing
    • Y-chromosome testing
      • Paternal inheritance
  • Mitochondrial sequencing
    • Control region
      • Maternal inheritance
  • Databasing
testing results
Testing Results
  • A full autosomal DNA profile consists of 15 polymorphic loci and 1 marker that is used to determine gender.
  • One multiplex amplification.
  • The probability of a random match in the general population is less than 1 in 1015.
  • There is no known link between these markers and disease.
dna databasing
DNA Databasing
  • Forensic DNA samples collected from crime scene evidence
  • DNA samples taken from convicted offenders
  • Missing persons
    • Unidentified human remains
    • Close relatives
    • Deduced victim knowns
      • Toothbrush
      • Hairbrush
what isn t put in a dna database
What isn’t put in a DNA Database?
  • Suspect profiles
    • Samples obtained by search warrant
    • Voluntary samples
  • Victim profiles
  • Elimination profiles
    • Husband/Boyfriend
missing persons
Missing Persons
  • Unidentified Human Remains
    • Searched against Forensic, Offender, Relatives, and Deduced Victim Knowns
  • Close Relatives
    • Searched against Unidentified Human Remains and Deduced Victim Knowns
    • Must have signed consent form on file
  • Deduced Victim Knowns
    • Searched against Forensic, Offender, Relatives, and Deduced Victim Knowns
mitochondrial dna testing
Mitochondrial DNA Testing
  • Short, circular DNA found in the cytoplasm
  • ~16kb in length
  • Control region is sequenced in two parts
    • HV1 and HV2
    • Results are compared to reference sequence
  • Family relationships along the maternal line.
    • Was it the Tsar’s family in the well?
    • Missing persons
mitochondrial dna testing1
Mitochondrial DNA Testing
  • FBI-Cooperative agreement
    • Accept cases from different states
  • Hairs, bones, teeth, etc.
    • Hair shafts
    • New and old bones
  • Maternal inheritance
  • “DNA of last resort”
    • If STR’s will not/did not work
summary
Summary
  • STR’s used in forensics have 4bp core repeat units. The DNA profile is determined by the number of repeats at each locus.
  • Databasing allows law enforcement agencies to share DNA information.
  • Y-Chromosome testing allows us to ignore the female components of mixtures.
  • Mitochondrial DNA testing can provide information from hair shafts, bones and teeth.