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Clinical Microscopy. “The Microscope”. Illuminator. Halogen Bulb Usually controlled by a rheostat (knob that controls the intensity). . Condenser. Compound lens system that focuses the light from the illuminator onto the specimen.

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clinical microscopy

Clinical Microscopy

“The Microscope”

illuminator
Illuminator
  • Halogen Bulb
  • Usually controlled by a rheostat (knob that controls the intensity).
condenser
Condenser
  • Compound lens system that focuses the light from the illuminator onto the specimen.
  • Gathers a large amount of light and condenses it into a small area.
aperture diaphragm
Aperture Diaphragm
  • Lever on the front of the condenser that allows you to control the amount of light leaving the condenser.
  • Opens and closes like the iris of you eye.
stage
Stage
  • Simply the place where the slide sits.
  • This area becomes very dirty. Keep it clean.
slide holder
Slide Holder
  • Mechanical device that holds the slide in place on the stage. Where ever the stage goes the slide goes!
objectives
Objectives
  • Traditionally come in four flavors in the lab
    • 10x, 40x, 50x, and 100x
  • Magnify the image
  • 50x and 100x require immersion oil.
    • The oil bends light waves so more light enters the objective
oculars
Oculars
  • Usually have a magnification power of 10x.
  • Form the final image.
  • Total Magnification: equals the oculars time the objectives. (40x times 10x = 400)
focus knobs
Focus Knobs
  • Two flavors of these:
    • Course
    • Fine
  • Some microscopes control the course and fine focus with one knob.
stage adjustment knobs
Stage Adjustment Knobs
  • Controls the stage movement.
  • Top knob controls forward and back movement.
  • Bottom knob controls left and right movement.
cleaning the microscope
Cleaning the Microscope
  • Keeping the microscope clean is extremely important.
  • Use a clean, lint-free lens tissue.
  • Don’t use a dry tissue to clean the lenses. Use a moist tissue first and then a dry one to mop up.

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cleaning
Cleaning...
  • Start by cleaning the illuminator.
  • Move to the condenser
  • Next clean the objectives, but clean the 100x last because there may be oil on it. Once you have cleaned the 100x, get a new tissue.

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cleaning15
Cleaning...
  • Clean the oculars
  • Lastly, wipe off the stage. Clean it last because it usually has the most dust and oil.
  • Make this a daily routine.

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