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Explore the intricate processes of photosynthesis within chloroplasts, from light-dependent reactions in thylakoids to the Calvin Cycle in the stroma. Learn how factors like water, temperature, and light intensity affect this vital plant function.
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The Reactions of Photosynthesis8-3 Flora Biology 1004
Inside the Chloroplast • Chloroplast contains thylakoid (sac-like membranes) • Thylakoids are arranged in stacks called “grana” • Area outside the thylakoids is called the “stroma” • Proteins in thylakoids organize chlorophyll/pigments into clusters known as photosystems • These are the light collection centers of the chloroplast.
2 Types of Reactions • Light Dependent • Light Independent • A.K.A. – Calvin Cycle
Light Dependent • Occurs in the thylakoids • Requires light • Converts ADP and NADP to ATP and NADPH • Water is split to form: • Oxygen gas (released) • 2 Hydrogen Ions • 2 electrons
Light Dependent Process • 1 – pigments in photosystem II absorb light • 2 – Energy in light is absorbed by electrons. • New electrons are provided by the splitting of water • 3 – High energy electrons move through ETC from photosystem II to photosystem I • 4 – Energy carried by electrons is used to pump Hydrogen ions from stroma to inner thylakoid • 5 – Pigments in Photosystem I use energy from light to re-energize electrons, NADP picks up these electrons and becomes NADPH.
Light Dependent Cont. • As more NADPH is made, more hydrogen is pumped into the inner membrane. • Outside becomes negatively charged, inside is positively charged – difference in charges provides energy to turn ADP into ATP (used in calvin cycle) • Hydrogen ions pass through membranes using ATP synthase – a protein
Calvin Cycle/Light Independent • Takes place in the stroma • During this process plants use the energy in ATP and NADPH (from dependent reactions) to build glucose • Does not require light • 6 CO2 molecules produce ONE, six-carbon sugar • Main product of Calvin Cycle is SUGAR
Process of Calvin Cycle • Six carbon dioxide molecules enter the cycle • Each molecule combines with six 5-carbon molecules producing 12 3-carbon molecules. • Each molecule then absorbs energy • 2 of molecules leave the cycle using them to produce sugars, lipids, amino acids, etc. • The remaining 10 molecules are converted back into 5-carbon molecules and then combine with 6 new carbon dioxide molecules to begin the next cycle.
The use of sugars made • The plant uses the sugars to meet its energy needs and to build more complex macromolecules such as cellulose that it needs for growth and development.
Factors Affecting Photosynthesis Rate • Shortage of Water • Temperature • Intensity of Light • Increase light intensity results in increase in the rate of photosynthesis.