estimation of carbohydrate by anthrone method l.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Estimation of Carbohydrate by Anthrone Method PowerPoint Presentation
Download Presentation
Estimation of Carbohydrate by Anthrone Method

Loading in 2 Seconds...

play fullscreen
1 / 5

Estimation of Carbohydrate by Anthrone Method - PowerPoint PPT Presentation


  • 1264 Views
  • Uploaded on

Estimation of Carbohydrate by Anthrone Method . The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates, either free or present in polysaccharides. . H 2 SO 4. Blue Green Complex. +. Anthrone. Furfural. * Principle. Materials.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Estimation of Carbohydrate by Anthrone Method' - iain


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
estimation of carbohydrate by anthrone method
Estimation of Carbohydrate by Anthrone Method
  • The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates, either free or present in polysaccharides.
principle

H2SO4

Blue Green

Complex

+

Anthrone

Furfural

* Principle
materials
Materials

1. Anthrone reagent (0.2% in conc. H2SO4 ).

2. Glucose (10mg/100ml).

3. Colorimeter or spectrophotometer.

slide4

60

5

55

10

50

45

15

40

20

35

25

30

Tube # Blank St. unk.

10 min.

Boiling Water Bath

Glucose (ml) 0 0.5 0.5

D. W. (ml) 1 0.5 0.5

Anthrone (ml) 4 4 4

Mix Well

COOL to Room Temperature

Read the Optical Density at 620 nm

calculations
* Calculations

Absorbance of Unknown Absorbance of Standard

=

Concentration of Unknown Concentration of Standard

CUn. = A Un. × C St.

A St.

Samar A. Damiati