Estimation of Carbohydrate by Anthrone Method . The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates, either free or present in polysaccharides. . H 2 SO 4. Blue Green Complex. +. Anthrone. Furfural. * Principle. Materials.
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1. Anthrone reagent (0.2% in conc. H2SO4 ).
2. Glucose (10mg/100ml).
3. Colorimeter or spectrophotometer.
Tube # Blank St. unk.
Boiling Water Bath
Glucose (ml) 0 0.5 0.5
D. W. (ml) 1 0.5 0.5
Anthrone (ml) 4 4 4
COOL to Room Temperature
Read the Optical Density at 620 nm
Absorbance of Unknown Absorbance of Standard
Concentration of Unknown Concentration of Standard
CUn. = A Un. × C St.
Samar A. Damiati