Chapter 11 Nucleotides and Nucleic Acids - PowerPoint PPT Presentation

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Chapter 11 Nucleotides and Nucleic Acids
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Chapter 11 Nucleotides and Nucleic Acids

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  1. Chapter 11 Nucleotides and Nucleic Acids

  2. The fundamental process of information transfer in cells

  3. 11.1 Nitrogenous Base

  4. The UV absorption spectra of the common ribonucleotides DNA OD260 / OD280 = 1.8 RNA OD260 / OD280 = 2.0

  5. 11.2 The pentoses of Nucleotides and Nucleic Acids

  6. 11.3 Nucleosides Are Formed by Joining a Nitrogenous Base to Sugar

  7. 11.4 Nucleotides Are Nucleosides Phosphates

  8. cAMP cGMP

  9. Formation of ADP and ATP

  10. Phosphoryl and pyrophosphoryl group transfer

  11. 11.5 Nucleic Acid Are Polynucleotides

  12. Shorthand notations for polynucleotide structures pA Cp ppU ppGpp cNMP GpApCpGpUpAp GACGUAp pGpApCpGpUpA GACGUA d-GACGTA

  13. 11.6 Classes of Nucleic Acids DNA RNA 11.7 Hydrolysis of Nucleic Acids 11.7.1 Hydrolysis by acid or alkali DNA apurinic acid RNA nucleoside 2`-and 3`-monophosphates

  14. 11.7.2 Enzymetic Hydrolysis nuclease RNase DNase

  15. endonuclease exonuclease

  16. The specificity of RNA hydrolysis by bovine pancteatic RNase

  17. Restriction enzymes recognize specific nucleotide sequences in double-strand DNA, that are usually four, five or six nucleotides long, and then cut both strands of the DNA at specific location. twofold axis of symmetry blunt end cohesive end 5`-overhang 3`-overhang

  18. nomenclature EcoRI genus namespecies name strain roman numeral

  19. A map showing the position of cut sites for a variety of restriction enzymes is called the restriction map for that DNA molecule.

  20. Restriction mapping of a DNA molecule