Chapter 11 Nucleotides and Nucleic Acids
The UV absorption spectra of the common ribonucleotides DNA OD260 / OD280 = 1.8 RNA OD260 / OD280 = 2.0
Shorthand notations for polynucleotide structures pA Cp ppU ppGpp cNMP GpApCpGpUpAp GACGUAp pGpApCpGpUpA GACGUA d-GACGTA
11.6 Classes of Nucleic Acids DNA RNA 11.7 Hydrolysis of Nucleic Acids 11.7.1 Hydrolysis by acid or alkali DNA apurinic acid RNA nucleoside 2`-and 3`-monophosphates
11.7.2 Enzymetic Hydrolysis nuclease RNase DNase
Restriction enzymes recognize specific nucleotide sequences in double-strand DNA, that are usually four, five or six nucleotides long, and then cut both strands of the DNA at specific location. twofold axis of symmetry blunt end cohesive end 5`-overhang 3`-overhang
nomenclature EcoRI genus namespecies name strain roman numeral
A map showing the position of cut sites for a variety of restriction enzymes is called the restriction map for that DNA molecule.