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This supplementary figure showcases the impact of ATG5 knockdown on LC3 protein levels in the study, using various experimental conditions and controls. The Western blot results display changes in LC3-I, LC3-II, and ATG5 protein expression, aiding in understanding autophagy regulation.
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Supplementary Figure 5 a T80 HEY ATG5 shRNA H2-1 + FAC ATG5 shRNA H2-2 + FAC ATG5 shRNA H2-1 + FAC ATG5 shRNA H2-2 + FAC ATG5 shRNA H2-1 ATG5 shRNA H2-2 Control-1 + FAC Control-2 + FAC ATG5 shRNA H2-1 ATG5 shRNA H2-2 Control-1 + FAC Control-2 + FAC Control-1 Control-2 Control-1 Control-2 17 kDa 17 kDa LC3-I LC3-I 12 kDa LC3-II LC3-II 12 kDa 76 kDa ATG5 76 kDa ATG5 38 kDa GAPDH 38 kDa GAPDH