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Enhancing Circadian Regulation with mPer1 E-box Tandem Repeats in Rat-1 Cells

To investigate circadian regulation, we incorporated wild-type and mutant E-boxes from the mPer1 gene in an SV40 basic promoter driving a dLuc reporter. The bioluminescence of transfected Rat-1 cells was monitored, showing alignment with mPer1 E(5)box SV40-dLuc. Our findings shed light on circadian control mechanisms.

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Enhancing Circadian Regulation with mPer1 E-box Tandem Repeats in Rat-1 Cells

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  1. Supplementary Figure We inserted three tandem repeats of wild-type and mutant E-boxes from the mPer1 gene, in front of the SV40 basic promoter driving a dLuc reporter, transfected the vector into Rat-1 cells and then monitored bioluminescence. The phase of SV40-driven dLuc reporters containing first E-box x3 and second E-box x3 was similar to that of mPer1 E(5)box SV40-dLuc (fifth E-box x3).

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