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kDa

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kDa

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  1. Supplementary Fig. 1. Expression and purification of Bacillus sp. BRC1 Bdh in/from E. coli. Lane 1, total cell lysates of E. coli BL21 harboring pET28a (control); lane 2, total cell lysates of E. coli BL21 harboring pET-bhd; lane 3, soluble fraction; lane 4, insoluble fraction; lane 5, purified Bdh (arrow). M 1 2 3 4 5 kDa 130 95 55 36 28 Supplementary Fig. 1

  2. Supplementary Fig. 2.Effects of pH (A) and temperature (B) on Bdhactivity toward the substrates D-2,3-BD (closed circles) and acetoin (open circles). A B Supplementary Fig. 2

  3. Supplementary Fig. 3. Enzyme kinetics of Bacillus sp. BRC1 Bdh. A, D-2,3-BD; B, Meso-2,3-BD; C, Acetoin; D, Diacetyl A B C D Supplementary Fig. 3

  4. Supplementary Fig. 4. Enzyme activity of Bdh from recombinant Bacillus sp. BRC1 strains. Closed bars, D-2,3-BD; open bars, acetoin. BRC1/pWH BRC1/pWH-bdh Xylose 5% Xylose 5% Xylose 20% Xylose 20% EFB hydrolysate EFB hydrolysate Supplementary Fig. 4