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This study evaluates the relative concentration of GST-SSB from Mpn using blue dextran for fractionation. The analysis was conducted with respect to OD280 measurements, targeting molecular weights around 55 kDa. Multiple eluted fractions were collected, with focus on specific volumes corresponding to their optical density readings. The data highlights the efficiency of various concentrations and their impact on the yield of GST-SSB, providing insight into optimal conditions for protein purification.
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A B 1 blue dextran GST-SSB Mpn SSB Mpn H10-SSB GST kDa M 55 Relative concentration (■) OD280 (♦) 0,5 40 35 cyt. C Mpn SSB 25 ova BSA 0 15 3 4 1 2 5 Eluted fraction (ml)