Gene silencing. Antigen-presenting cell (APC). ( 2) Phagosomal escape ・ Proton sponge effect of dendrimer ・ Disruption of phagosome membrane by a -CyD. (1) MR-mediated phagocytosis. ・ Enhancement of recognition ability by MR. Mannose receptor (MR). Phagosome. Lysosome.
Antigen-presenting cell (APC)
(2) Phagosomal escape
・Proton sponge effect of dendrimer
・Disruption of phagosome membrane by a-CyD
(1) MR-mediated phagocytosis
・Enhancement of recognition ability by MR
(3)Localization in cytoplasm
(4)Dissociation of siRNA
: Dendrimer (G3)
Supplementary Figure 5. Proposed scheme for APC-selective RNAi efficiency of Man-S-a-CDE (G3, DSM4)/siRNA complex.