Hey Ladies, its quiz time!

1. Hey Ladies, its quiz time! Boggusrl@email.uc.edu

2. How Rachel is going to do this • Put up a slide • Write down the answer • Go over it immediately • Repeat many many times • 113 slides of adventure await!! Did you remember your party hats????

3. What type of epithelium is this? (how do you know?)

4. Transitional Epithelium • How do you know? • Scalloped appearance • Bi-nucleated cells

5. What is the predominant type of tissue? (how do you know?)

6. Smooth Muscle • How do you know? • Nuclei more centrally located • Seen here in both planes of section • In cross section you can tell its smooth muscle because of the centrally located nuclei but also because some cells do not have the nucleus cut in the plane so look anucleate and also because of the varying thicknesses of the cuts.

7. What is this?How do you know?

8. Peripheral Nerve • How do you know? • Encased by an epineurium • Wavy/bubbly appearance of the nerve fibers within epineurium REMEMBER: CIRCLE OF WAVY SHIT

9. What is the arrow pointing to?

10. Serous Demilune • How did you know? • Because most of it looks like a mucous secreting gland (vacuolated cytoplasm with laterally displaced nuclei) But the crescent shaped rim (hence LUNE=Moon!) is more like a serous gland (basophilic) so it’s a serous demilune

11. What is this? How do you know?

12. Dense Irregular Connective Tissue • How do you know? • Few nucleated cells - fibroblasts • Lots of collagen arranged random-like • Nuclei located outside of the collagen

13. What is the predominant tissue?

14. Brown (multilocular) fat • How do you know? • Bubbly appearance signifies many fat-filled vesicles • Nuclei still laterally displaced • Remember that you have multilocular fat as a stage of differentiation to regular white fat.

15. What type of tissue?how do you know?

16. Fibrocartilage • How did you know? • No perichondrium evident • Cells located in lacunae • Remember that fibrocartilage is often continuous with surrounding CT.

17. What process is occurring in this slide?

18. Intramembranous Bone Formation • How did you know? • Osteoblasts surrounding osteoid with osteocytes enclosed in the osteoid…

19. What type of epithelium?

20. Ciliated pseudostratified columnar epithelium with goblet cells • How did you know? • Because Dr. Michaels drilled this into your brains? • NO! (well ok, probably) but also because you can sort of see that all the cells maintain their connection with the basal lamina and you can clearly see goblet cells and cilia

21. What is the tissue?

22. Cardiac muscle • How did you know? • Centrally located nuclei surrounded by large ring of cytoplasm • For the most part, more uniformly shaped cuts • Compare to smooth muscle cut in cross section—different sizes of section and nuclei not present as often in the cut.

23. Which of the following are Transmission Electron micrographs? And what are the EMs of? C. D. A. B.

24. A, C, D • REMEMBER THAT FREEZE FRACTURE EMS ARE VIEWED WITH TRANSMISSION EM! • All of them are cillia • Remember the basal bodies (what is the sydrome when you don’t have dynein arms? • Kartageners syndrome • What was used to obtain image B? • Scanning EM

25. What is indicated by the red arrow?

26. Glycogen • How did you know • Because that is what glycogen looks like on the EM • Compare to collagen cut in cross section • Remember that glycogen particles aggregate and also that they exist OVER other stuctures, usually SER

27. What is this?

28. Golgi Apparatus • Layered like apperance • Which end is trans/cis?

29. What is this? How do you know?

30. Smooth Muscle cell • How did you know? • Presence of dense bodies and dense plaques as well as caveolin (flask shaped invaginations of the membrane)

31. What is this?

32. ganglion • How did you know? • Bullseye like appearance of the cells. FRIED EGGS

33. What are indicated by the green arrows?

34. Osteoclasts • How did you know? • Multinucleated cells • Found in indented areas of bone (know its bone because you can see the osteocytes in lacunae (yellow arrows)

35. Ignore the arrowWhat is predominate on this slide?

36. Serous glands • How did you know? • Because it looks glandular and there is still color in the cytoplasm. Compare to mucous secreting glands, which have vacuolated cytoplasm

37. What is indicated by the number 1? 2?

38. 1) Bone Marrow 2) spongy/cancellous bone spicule • How do you know? • 1) you know it is bone marrow because it is found between trabeculae of bone (bright red) and it is rich in lipid and hematopoetic cells (purple) Ok, so you haven’t had this yet, I’m just preparing you for next block. • 2) you know its cancellous bone because you can see the osteocytes in lacunae

39. Label A-D ____A__________B___________C____________D______

40. A) Zone of resting cartilage • B) Zone of proliferation • C) Zone of hypertrophy • D) Zone of calcification • What process was indicated? • Endochondrial bone formation

41. What is along the lumen?

42. stereocilia • You know because it branches and it is long • Another clue – stereocilia often in epididymis. You don’t know what this looks like yet but it is like 35148743 small tubes like this. ALWAYS stereocilia here

43. What is this?

44. Loose Irregular Connective Tissue • You know because there is a general lack of nuclei and very sparse and randomly placed collagen

45. What is indicated by the arrow?

46. Mast Cells • You can tell because it is purple because of metochromasia • And if you look really closely you can see that it is a granule-filled cell.

47. What is this?

48. Dense Regular Connective Tissue • How did you know? • Wavy appearance of collagen • Few cells (fibroblasts) located outside of the collagen fibers

49. What is indicated by the arrow?

50. Node of Ranvier • You know because it is stained for myelin and the indentation  place without myelin • What does myelin do? • What forms it?