Polar lipids of strain DSM 45459 (H32
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Polar lipids of strain DSM 45459 (H32 T ). DPG. GL1. PL1. PG. GL2. PL2. PC. GL3. PI.

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Polar lipids of strain dsm 45459 h32 t

Polar lipids of strain DSM 45459 (H32T)

DPG

GL1

PL1

PG

GL2

PL2

PC

GL3

PI

Supplementary Fig. S1b. Two-dimensional thin-layer chromatograms of phospholipids of H32T. The chromatographic conditions were as follows. Silica Gel 60 thin-layer plates (10 by 10 cm) were spotted with 15 μl of a whole-cell lipid extract.

Chloroform/methanol/water (65:25:4, by vol) was used in the first direction, and chloroform/acetic acid/methanol/water (80:18:12:5, by vol) was used in the second direction. Plates were sprayed with molybdatophosphoric acid reagent to locate and differentiate the phospholipids.

Abbreviations: DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PC, phosphatidylcholine; PI, phosphatidylinositol; PL, unknown phospholipid (2x); GL, unknown glycolipid (3x).