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Enhancement of l-Cysteine Production in Disrupted gshA Gene Strain

This study investigates the l-cysteine and l-cystine production in a gshA gene disrupted strain compared to the wild type strain, using cultivation in SM1 medium with 30 mM Na2S2O3 and thiosulfate for 36 hours at 30°C. The concentration of l-cysteine and l-cystine was analyzed using the Gaitonde method. Results show means and standard deviations from three independent experiments.

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Enhancement of l-Cysteine Production in Disrupted gshA Gene Strain

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  1. Supplemental Figure 1 Supplemental Figure 1l-cysteine plus l-cystineproduction in l-cysteine producer disrupted gshA gene WT (BW25113) harboring pDES (closed circles) and DgshA harboring pDES (opened circles) were cultivated in SM1 medium with 30 mMNa2S2O3 for 36 hours at 30°C. These strains were cultivated in SM1 medium with 30 mMthiosulfate for 36 hours at 30°C. The concentration of l-cysteine plus l-cystine was determined by Gaitonde method. Values indicate means and standard deviations of results from three independent experiments.

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