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Isopropylmalate Synthase

Isopropylmalate Synthase. Amino acid analogs. Amino Acid Analogs. Analogs – resemble naturally occurring amino acids Inhibit Wild Type – Why? What happens if incorporated into protein? Why are normal amino acids not synthesized? . Expose Wild Type Salmonella to Fluoroleucine. Observed.

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Isopropylmalate Synthase

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  1. Isopropylmalate Synthase Amino acid analogs

  2. Amino Acid Analogs Analogs – resemble naturally occurring amino acids Inhibit Wild Type – Why? What happens if incorporated into protein? Why are normal amino acids not synthesized?

  3. Expose Wild Type Salmonella to Fluoroleucine Observed Expected Minimal Media containing Fl-leu What is nature of variants that grow?

  4. Salmonella Strain ComparisonGrown on regular Minimal Media Isopropyl Malate Synthase – First committed enzyme in the synthesis of leucine

  5. Nature of Mutants

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