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Analysis of Isoform Variability and Splice Patterns Across Populations

This study examines isoform expression variability at different start points for all populations, focusing on the number of isoforms produced and the splice deltas, which represent the difference in base pairs from the major expressed splice pattern. Special attention is given to spliced reads that begin before the first exon (farStart) and extend beyond the last exon (farEnd). The findings highlight the complexity of splice junctions and their categorization, revealing insights into alternative splicing mechanisms that may influence gene function and regulation.

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Analysis of Isoform Variability and Splice Patterns Across Populations

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  1. Isoforms / startpointfor all populations numberofisoforms per startpoint for all populations

  2. splice-deltas (i.e. difference [in bp] from the major expressed splice-pattern)

  3. farStart: spliced read that starts before the first exon farEnd: spliced read that extends beyond the last exon a splice-junction may be in multiple categories

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