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PHYLOGENETIC ANALYSIS OF A NEW GROUP OF CUCUMBER MOSAIC VIRUS ISOLATES BASED ON 2B GENE SEQUENCING

PHYLOGENETIC ANALYSIS OF A NEW GROUP OF CUCUMBER MOSAIC VIRUS ISOLATES BASED ON 2B GENE SEQUENCING Nesmelov I.B., Gnutova R.V., Tolkach V.Ph. History of question. was described for the first time by Selby at 1903

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PHYLOGENETIC ANALYSIS OF A NEW GROUP OF CUCUMBER MOSAIC VIRUS ISOLATES BASED ON 2B GENE SEQUENCING

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  1. PHYLOGENETIC ANALYSIS OF A NEW GROUP OF CUCUMBER MOSAIC VIRUS ISOLATES BASED ON 2B GENE SEQUENCING Nesmelov I.B., Gnutova R.V., Tolkach V.Ph.

  2. History of question • was described for the first time by Selby at 1903 • The 60 strains of this virus are infecting about 500 plant species from 67 families in nature • CMV strains are divided into two groups according to the differences in their genomes and biological features • More than 40 isolates was described at Primorye region • On the base of serological assay isolates from Primorye were ascribed to Far Eastern serotype • at 2008 orchid isolates was ascribed to Eastern Asian isolates

  3. Aim The aim of our work is to show are the examined orchid isolates came from abroad or they are belonging to natural Far-Eastern CMV population

  4. Virus structure • spherical form • size~29 nm • 32 morphological subunits of coat protein innodes of crystal lattice • contain 3 molecules of (+) RNA

  5. Methods Infected plant Nicotiana tobacumcv Xanthi Isolation by Dreyper (1991) Total RNA RT-PCR was made by “Sintol” company Sequencing

  6. Phylogenetic analysis South Korea China India East-Asian isolates China Far-Eastern China

  7. Genetic distances Jp – Japan, SK – South Korea, Eur – Europe, Ch – China, Ind – india, FE – Far-Eastern isolates

  8. Conclusion At present time we have separate population of CMV on orchid plants on Far-Eastern territory of Russian Federation. On both genetic distances and phylogenetic reconstruction Far-Eastern isolates are part of East-Asian Isolates.

  9. Thanks for your attention

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